Sequence Analysis of the Ribosomal Internal Transcribed Spacers Region in Psocids (Psocoptera: Liposcelididae) for Phylogenetic Inference and Species Discrimination
Sequence Analysis of the Ribosomal Internal Transcribed Spacers Region in Psocids (Psocoptera: Liposcelididae) for Phylogenetic Inference and Species Discrimination
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用于系统发育推断和物种歧视的 Psocids（Psocoptera：Liposcelididae）核糖体内部转录间隔区的序列分析
DOI：
10.1603/ec11177
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发表时间：
2011-10-01
影响因子：
2.2
通讯作者：
Wang, Jin-Jun
中科院分区：
文献类型：
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作者：
Wei, Dan-Dan;Yuan, Ming-Long;Wang, Jin-Jun
姬蜂科(Pocoptera：Liposcelidae：Liposcelis spp.)是储粮的主要害虫，通常发生在各种储藏产品上。由于以储存的谷物、污染的食品和农产品以及传播包括真菌和细菌在内的有害微生物为食，越来越多的人认识到脂胞杆菌属的重要性。瓢虫在形态上有很大的相似之处，通常在同一生态系统中出现。因此，进一步实施种群研究的第一步是基于分子方法的物种的准确鉴定。在本研究中，我们测定了中国所在的16个地点的6个种的100个个体的核rDNA内转录间隔区(ITS)1-5.8S-ITS2区的核苷酸序列。我们评价了该标记在脂胞属物种系统发育推断研究中的适用性。我们还建立了一种基于ITS2序列的六种磷脂细菌的分子鉴定方法。结果表明，ITS1-5.8S-ITS2序列是研究脂藻属种群遗传和系统发育的有用工具。本研究的结果表明，ITS2序列可以作为一种可靠的工具来区分所测试的6个种。此外，当在不同地理人群中进行测试时，所述的多重方法被证明是可靠的。
Psocids (Psocoptera: Liposcelididae: Liposcelis spp.) are major pests of stored grain and commonly occur on a wide range of stored products. Increasingly, the genus of Liposcelis has gained recognition of their importance due to their feeding on stored grains, contaminating food, and agricultural commodities as well as transmitting harmful microorganisms, including fungi and bacteria. Psocids have close morphological similarities and often commix occur at the same ecosystems. Therefore, a first step necessary to further implement population studies is the accurate identification of species, based on molecular methods. In this study, we determined nucleotide sequences of the nuclear rDNA internal transcribed spacer (ITS) 1-5.8S-ITS2 region in 100 individuals of six Liposcelis species (including Liposcelis bostrychophila Badonnel, Liposcelis entomophila (Enderlein), Liposcelis decolor (Pearman), Liposcelis tricolor Badonnel, Liposcelis paeta Pearman, and Liposcelis yunnaniensis Li & Li) from 16 locations of China. We evaluated the suitability of this marker for phylogenetic inference study in the Liposcelis species. We also developed a molecular identification method for six Liposcelis species based on ITS2 sequence. Results demonstrate that ITS1-5.8S-ITS2 sequences are a useful tool for the population genetic study and phylogeny estimation of Liposcelis species. The results of this study indicate that the ITS2 sequences can be a reliable tool for species discrimination of the six species of psocids tested here. In addition, the multiplex method described proved reliable when tested across different geographical populations.