Structural Studies of an RNA Aptamer Containing GGA Repeats Under Ionic Conditions Using Microchip Electrophoresis, Circular Dichroism, and 1D-NMR
Structural Studies of an RNA Aptamer Containing GGA Repeats Under Ionic Conditions Using Microchip Electrophoresis, Circular Dichroism, and 1D-NMR
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DOI：
10.1089/oli.2008.0167
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发表时间：
2009-06-01
期刊：
影响因子：
--
通讯作者：
Nishikawa, Satoshi
中科院分区：
文献类型：
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作者：
Nishikawa, Fumiko;Murakami, Kazuyoshi;Nishikawa, Satoshi
核磁共振（NMR）研究表明，具有GGA重复序列的RNA/DNA寡聚体在K+或Na+离子存在下含有独特的G-四链体结构。在这项研究中，我们使用微芯片电泳研究的RNA适体的结构，对牛朊病毒蛋白，具有四个GGA-三联体重复序列（wt 2）。我们分析了适体的结构变化并表征了适体的二聚体形成。突变，圆二色性，和一维NMR研究的WT 2显示，K+离子诱导WT 2承担一个热稳定的二聚体在一个分子内的G-四链体与平行取向。
Nuclear magnetic resonance (NMR) studies have shown that RNA/DNA oligomers with GGA repeat sequences contain unique G-quadruplex structures in the presence of K+ or Na+ ions. In this study, we used microchip electrophoresis to study the structure of an RNA aptamer against bovine prion protein that possessed four GGA-triplet repeats (wt2). We analyzed the structural changes and characterized dimer formation of the aptamer. Mutational, circular dichroism, and one-dimensional NMR studies of wt2 revealed that K+ ions induce wt2 to assume a thermostable dimer in an intramolecular G-quadruplex with parallel orientation.