Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy
Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy
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DOI：
10.1364/ol.30.000468
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发表时间：
2005-03-01
期刊：
影响因子：
3.6
通讯作者：
Depeursinge, C
中科院分区：
文献类型：
--
作者：
Marquet, P;Rappaz, B;Depeursinge, C
我们已经开发出一种数字全息显微镜（DHM），在传输模式，特别是致力于定量可视化的阶段对象，如活细胞。该方法是基于在别处详细介绍的原始数值算法[Cucche等人，38，6994（1999）]。DHM图像的活细胞培养显示什么是我们的知识的第一次。它们表示光路长度在整个单元上的分布，其已经以亚波长精度测量。这些DHM图像进行了比较，通过使用广泛使用的相位衬度和Nomarski微分干涉衬度技术。(C)2005年，美国光学学会。
We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsewhere [Cuche et al., Appl. Opt. 38, 6994 (1999)]. DHM images of living cells in culture are shown for what is to our knowledge the first time. They represent the distribution of the optical path length over the cell, which has been measured with subwavelength accuracy. These DHM images are compared with those obtained by use of the widely used phase contrast and Nomarski differential interference contrast techniques. (C) 2005 Optical Society of America.