Implantation increases tensile strength and collagen content of self-assembled tendon constructs
Implantation increases tensile strength and collagen content of self-assembled tendon constructs
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DOI：
10.1152/japplphysiol.00921.2009
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发表时间：
2010-04-01
影响因子：
3.3
通讯作者：
Arruda, Ellen M.
中科院分区：
文献类型：
--
作者：
Calve, Sarah;Lytle, Ian F.;Arruda, Ellen M.
小牛S，莱特尔如果，格罗什K，布朗DL，阿鲁达EM。植入可增加自组装肌腱结构的抗拉强度和胶原含量。应用生理学杂志108：875-881,2010。2010年1月28日首次出版；doi:10.1152/japplphysiol.00921.2009.-Tissue-engineered肌腱来源于自体细胞来源，具有提供理想的替代移植物的潜力，该移植物具有生物兼容性，并能够适应体内环境的特定机械要求。无支架肌腱构建已经在体外成功地被设计出来。然而，与天然肌腱相比，构建的肌腱显示出较低的抗拉强度和胶原含量。我们假设体外环境缺乏某些环境刺激，体内植入将促进工程化组织的成熟。利用成年大鼠的跟腱成纤维细胞，在体外建立了自组织结构。将肌腱构建物植入成年大鼠腹股沟皮下4wk，而对照组仍在体外。与体外对照相比，植入的结构的硬度增加了三个数量级(7,500比22.3千帕)。切线模数的增加与胶原含量的显著增加有关，根据羟脯氨酸浓度的测量，从体外对照组的3.9%到体内条件组的22.7%。此外，体内植入后，胶原纤维直径从22.0 nm增加到75.4 nm。体内条件构建物的抗张强度和胶原含量与新生大鼠胫骨前肌腱的测定值相似。
Calve S, Lytle IF, Grosh K, Brown DL, Arruda EM. Implantation increases tensile strength and collagen content of self-assembled tendon constructs. J Appl Physiol 108: 875-881, 2010. First published January 28, 2010; doi:10.1152/japplphysiol.00921.2009.-Tissue-engineered tendons, derived from an autologous cell source, have the potential to provide an ideal replacement graft that is biologically compatible and has the ability to adapt to the specific mechanical requirements of the in vivo environment. Scaffold-free tendon constructs have been successfully engineered in vitro. However, when compared against native tendons the constructs demonstrate both a lower tensile strength and collagen content. We hypothesized that the in vitro environment lacks certain environmental stimuli and that implantation in vivo would facilitate the maturation of engineered tissues. Using primary Achilles tendon fibroblasts from adult rats, self-organizing constructs were created in vitro. Tendon constructs were implanted subcutaneously into the groins of adult rats for 4 wk, while controls remained in vitro. Implanted constructs increased in stiffness by three orders of magnitude when compared with the in vitro controls (7,500 vs. 22.3 kPa). This increase in tangent modulus correlated with a significant increase in collagen content, as measured by hydroxyproline concentration, from 3.9% for the in vitro controls to 22.7% in the in vivo conditioned group. In addition, collagen fiber diameter increased from 22.0 to 75.4 nm as a result of in vivo implantation. The tensile strength and collagen content of in vivo conditioned constructs were similar to the values determined for neonatal rat tibialis anterior tendons.