Interaction between clenbuterol and run training: Effects on exercise performance and MLC isoform content
Interaction between clenbuterol and run training: Effects on exercise performance and MLC isoform content
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DOI：
10.1152/jappl.1996.80.3.795
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发表时间：
1996-03-01
影响因子：
3.3
通讯作者：
Smith, SB
中科院分区：
文献类型：
--
作者：
Ingalls, CP;Barnes, WS;Smith, SB
本研究的目的是确定克伦特罗(CB)给药和间歇训练对小鼠骨骼肌运动成绩和肌球蛋白轻链(MLC)亚型表达的单独和联合影响。将小鼠随机分为4组：1)对照组(Con)，2)运动组(Ex)，3)药物组(CB)，或4)运动+药物组(Ex+CB)。CB和Ex+CB小鼠灌胃CB(1.6 mg/kg)4d/wk。EX和Ex+CB小鼠在机动跑台上训练4d/wk(3组，3min，36~40m/min，10~17%级，30-S恢复)。在8wk的治疗后，运动调节使Ex组的总作业量增加了58%，而CB组的总作业量减少了25%；与运动训练相结合，CB组消除了Ex引起的作业量增加。聚丙烯酰胺凝胶电泳法显示，跑步训练、CB治疗或两者结合并不能促进比目鱼肌、腓肠肌或胫骨前肌中快、慢MLC亚型的改变。虽然在8wk后彼此之间没有差别，但CB和Ex+CB处理的下列变量的值显著高于CON和Ex：肌肉质量(17-46%)、总蛋白(22-50%)和肌原纤维蛋白(19-53%)。结果表明，CB降低运动能力，而Ex和CB的联合作用对跑步成绩有拮抗作用；两种处理不会相互作用来减弱CB对骨骼肌的合成代谢作用，也不会改变MLC亚型。
The purpose of this study was to determine the separate and combined effects of clenbuterol (CB) administration and interval training on running performance and myosin light-chain (MLC) isoform expression in mouse skeletal muscle. Mice were randomly assigned to one of four treatment groups: 1) control (Con), 2) exercise (Ex), 3) drug (CB), or 4) exercise + drug (Ex+CB). CB and Ex+CB mice were given CB (1.6 mg/kg) orally 4 days/wk. Ex and Ex+CB mice were trained 4 days/wk on a motorized treadmill (3 sets of 3 min, 36-40 m/min, 10-17% grade, 30-s recovery). After 8 wk of treatment, exercise conditioning increased total work performed 58% in the Ex group during a run-to-exhaustion treadmill test, whereas CB decreased total work by 25% in the CB group; in combination with exercise training, CB treatment eliminated the Ex-induced increase in work. Polyacrylamide gel electrophoresis indicated that run training, CB treatment, or a combination did not (P > 0.01) promote changes in fast and slow MLC isoforms in the soleus, gastrocnemius, or tibialis anterior muscles. Although not different from each other after 8 wk, CB and Ex+CB treatments produced significantly greater values than Con and Ex for the following variables: muscle mass (17-46%), total protein (22-50%), and myofibrillar protein (19-53%). It was concluded that CB decreases exercise performance and that the combination of Ex and CB have antagonistic effects on running performance; the two treatments do not interact to diminish the anabolic effects of CB on skeletal muscle and do not alter MLC isoform profiles.