CLE40 Signaling Regulates Root Stem Cell Fate1
CLE40 Signaling Regulates Root Stem Cell Fate1
复制标题
DOI：
10.1104/pp.19.00914
复制
发表时间：
2020-04-01
期刊：
影响因子：
7.4
通讯作者：
Simon, Ruediger
中科院分区：
文献类型：
--
作者：
Berckmans, Barbara;Kirschner, Gwendolyn;Simon, Ruediger
柱状细胞的命运是由独立于WUSCHEL相关HOMEOBOX 5的因子控制的，CLAVATA 3/ESR-RELATED 40肽的主要作用是调节静止中心的活性和位置。拟南芥（Arabidopsis thaliana）根分生组织的静止中心（QC）作为组织者，促进相邻细胞中的干细胞命运并形成周围干细胞生态位。QC远端的干细胞，即鼻小柱干细胞（CSC），通过QC表达的转录因子WUSCHEL相关HOMEOBOX 5（WOX 5）维持在未分化状态，并产生鼻小柱细胞。分化的鼻小柱细胞通过分泌肽CLAVATA 3/ESR-RELATED 40（CLE 40）提供反馈信号，肽CLAVATA 3/ESR-RELATED 40（CLE 40）通过受体激酶ARABIDOPSIS CRINKLY 4（ACR 4）和CLAVATA 1（CLV 1）起作用以控制WOX 5表达。以前，有人提出W 0X 5蛋白从QC移动到CSC中是CSC维持所需的，并且CLE 40/CLV 1/ACR 4信号传导模块限制W 0X 5移动性或功能。在此，通过探索CLE 40/CLV 1/ACR 4在CSC维持中的功能来验证这些假设。然而，CLE 40/CLV 1/ACR 4在限制WOX 5或其他荧光测试蛋白的迁移率中没有作用。此外，与先前的观察结果相反，W 0X 5迁移率不需要抑制CSC分化。我们认为WOX 5主要在QC中起作用，在QC中产生其他短程信号，不仅抑制分化，而且促进相邻细胞中的干细胞分裂。因此，柱衍生的CLE 40信号的主要功能是通过远端结构域中的ACR 4/CLV 1受体抑制QC特异性基因表达并通过近端分生组织中的CLV 2受体促进WOX 5表达来将QC定位在距根尖限定的距离处。
Columella stem cell fate is controlled by factors acting independently of WUSCHEL RELATED HOMEOBOX 5 and the main role of the CLAVATA3/ESR-RELATED 40 peptide is the regulation of quiescent center activity and position.The quiescent center (QC) of the Arabidopsis (Arabidopsis thaliana) root meristem acts as an organizer that promotes stem cell fate in adjacent cells and patterns the surrounding stem cell niche. The stem cells distal from the QC, the columella stem cells (CSCs), are maintained in an undifferentiated state by the QC-expressed transcription factor WUSCHEL RELATED HOMEOBOX5 (WOX5) and give rise to the columella cells. Differentiated columella cells provide a feedback signal via secretion of the peptide CLAVATA3/ESR-RELATED40 (CLE40), which acts through the receptor kinases ARABIDOPSIS CRINKLY4 (ACR4) and CLAVATA1 (CLV1) to control WOX5 expression. Previously, it was proposed that WOX5 protein movement from the QC into CSCs is required for CSC maintenance, and that the CLE40/CLV1/ACR4 signaling module restricts WOX5 mobility or function. Here, these assumptions were tested by exploring the function of CLE40/CLV1/ACR4 in CSC maintenance. However, no role for CLE40/CLV1/ACR4 in constricting the mobility of WOX5 or other fluorescent test proteins was identified. Furthermore, in contrast to previous observations, WOX5 mobility was not required to inhibit CSC differentiation. We propose that WOX5 acts mainly in the QC, where other short-range signals are generated that not only inhibit differentiation but also promote stem cell division in adjacent cells. Therefore, the main function of columella-derived CLE40 signal is to position the QC at a defined distance from the root tip by repressing QC-specific gene expression via the ACR4/CLV1 receptors in the distal domain and promoting WOX5 expression via the CLV2 receptor in the proximal meristem.