Characterisation and comparison of immune response mechanisms in an indigenous and a commercial pig breed after classical swine fever vaccination
Characterisation and comparison of immune response mechanisms in an indigenous and a commercial pig breed after classical swine fever vaccination
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DOI：
10.1111/age.13152
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发表时间：
2021-11-02
期刊：
影响因子：
2.4
通讯作者：
Mishra, B. P.
中科院分区：
文献类型：
--
作者：
Mehrotra, A.;Bhushan, B.;Mishra, B. P.
猪瘟减毒活疫苗已成功地用于预防和控制猪瘟爆发60年。然而，对疫苗的免疫反应机制仍然知之甚少。此外，很少有关于对脑脊液疫苗反应的品种差异的报道。在这项研究中，我们在接种脑脊液前和接种脑脊液后7天分别生成了本地Ghurrah和商品长白猪品种的外周血单个核细胞转录组。随后，进行了品种间和品种内差异基因表达分析。结果显示，接种疫苗7天后，两个品种的外周血单核细胞转录组谱存在很大差异。接种前，基因集富集分析显示，Ghurrah基因中富集了与抗原感知和先天免疫应答相关的途径，而长白基因中富集了与适应性免疫相关的途径。与接种疫苗后的长白猪相比，古拉猪表现出更大的免疫调节作用。在Ghurrah中，接种疫苗后细胞周期过程和t细胞反应途径上调。然而，接种疫苗后，没有途径在长白猪中上调。接种疫苗后，两个品种的炎症相关通路均下调。在接种疫苗后，这两个品种的炎症关键调节因子如IL1A、IL1B、NFKBIA和TNF基因均被强烈下调。总的来说，我们的研究结果阐明了两个不同品种的宿主免疫应答CSF疫苗的机制，并揭示了在疫苗的全球免疫应答中起重要作用的共同关键基因。
The live attenuated classical swine fever (CSF) vaccine has been successfully used to prevent and control CSF outbreaks for 6 decades. However, the immune response mechanisms against the vaccine remain poorly understood. Moreover, very few reports exist regarding the breed differences in the response to CSF vaccine. In this study, we generated the peripheral blood mononuclear cell transcriptomes of indigenous Ghurrah and commercial Landrace pig breeds, before and 7 days after CSF vaccination. Subsequently, between and within-breed differential gene expression analyses were carried out. Results revealed large differences in pre-vaccination peripheral blood mononuclear cell transcriptome profiles of the two breeds, which were homogenised 7 days after vaccination. Before vaccination, gene set enrichment analysis showed that pathways related to antigen sensing and innate immune response were enriched in Ghurrah, while pathways related to adaptive immunity were enriched in Landrace. Ghurrah exhibited greater immunomodulation compared to Landrace following the vaccination. In Ghurrah, cell-cycle processes and T-cell response pathways were upregulated after vaccination. However, no pathways were upregulated in Landrace after vaccination. Pathways related to inflammation were downregulated in both the breeds after vaccination. Key regulators of inflammation such as IL1A, IL1B, NFKBIA and TNF genes were strongly downregulated in both the breeds after vaccination. Overall, our results have elucidated the mechanisms of host immune response against CSF vaccination in two distinct breeds and revealed common key genes instrumental in the global immune response to the vaccine.