Splenic CD19-CD35+B220+ cells function as an inducer of follicular dendritic cell network formation
Splenic CD19-CD35+B220+ cells function as an inducer of follicular dendritic cell network formation
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DOI：
10.1182/blood-2007-01-068387
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发表时间：
2007-08-15
期刊：
影响因子：
20.3
通讯作者：
Ohno, Hiroshi
中科院分区：
文献类型：
--
作者：
Murakami, Takaya;Chen, Xin;Ohno, Hiroshi
滤泡树突状细胞（Follicular dendritic cells，FDC）在淋巴滤泡中形成网状FDC网络，在二次免疫应答中以抗原-抗体免疫复合物（antigen-antibody immune complex，IC）的形式将天然抗原保留和呈递给B细胞中发挥重要作用。虽然已经假设存在FDCs的迁移前体，但其实体尚未阐明。在这里，我们报告了小鼠脾脏CD 19（-）CD 11 c（-）CD 35（+）B220（+）细胞作为FDC网络形成诱导剂的鉴定。我们证明，CD 19（-）CD 11 c（-）CD 35（+）B220（+）细胞与基质细胞一起具有形成淋巴滤泡样结构的显著能力，该结构包含最初来源于CD 35（+）网状组织中的CD 19（-）CD 11 c（-）CD 35（+）B220（+）细胞的B220（+）FDC（-）M1（+）网状细胞。我们的研究结果表明，CD 19（-）CD 11 c（-）CD 35（+）B220（+）细胞作为FDC网络形成的诱导剂，并且CD 19（-）CD 11 c（-）CD 35（+）B220（+）细胞和基质细胞之间的相互作用是启动淋巴滤泡形成所必需的。
Follicular dendritic cells (FDCs) form a reticular FDC network in the lymphoid follicle that is essential for the retention and presentation of native antigens in the form of antigen-antibody immune complexes (ICs) to B cells during secondary immune response. Although the presence of migrating precursors of FDCs has been hypothesized, their entity has not been elucidated. Here we report the identification of murine splenic CD19(-)CD11c(-)CD35(+)B220(+) cells as an inducer of FDC network formation. We demonstrated that CD19(-)CD11c(-)CD35(+)B220(+) cells, together with stromal cells, had the remarkable ability to form lymphold-follicle-like structures that contained B220(+)FDC(-)M1(+) reticular cells originally derived from CD19(-)CD11c(-)CD35(+)B220(+) cells in the CD35(+) reticulum. Our results indicate that CD19(-)CD11c(-)CD35(+)B220(+) cells function as an inducer of FDC network formation and that the interaction between CD19(-)CD11c(-)CD35(+)B220(+) cells and stromal cells is required to initiate lymphoid follicle formation.