Inhibitory effect of natural metal ion chelators on the autolysis of sea cucumber (Stichopus japonicus) and its mechanism
Inhibitory effect of natural metal ion chelators on the autolysis of sea cucumber (Stichopus japonicus) and its mechanism
复制标题
天然金属离子螯合剂对海参自溶的抑制作用及其机制
DOI：
10.1016/j.foodres.2020.109205
复制
发表时间：
2020
影响因子：
8.1
通讯作者：
Fereidoon Shahidi
中科院分区：
文献类型：
--
作者：
Zi-qiang Liu;Da-yong Zhou;Yu-xin Liu;Man-man Yu;Bing Liu;Liang Song;Xiu-ping Dong;Hang Qi;Fereidoon Shahidi
以草酸和茶多酚为天然金属离子螯合剂，对活海参进行了贮藏试验。这些螯合剂对自溶现象的抑制作用和潜在的作用机制进行了研究，第一次通过使用扫描电子显微镜，差示扫描量热法，低场核磁共振和共聚焦激光扫描显微镜。外界刺激通过从细胞释放钙离子（Ca 2+）到细胞外结缔组织中引起自溶，启动海参体壁（SCBW）中的基质金属蛋白酶（MMP）的活性。MMP随后降解微原纤维网络（支持相互连接的胶原纤维和连接胶原纤维的原纤维间蛋白聚糖桥），以释放限制在胶原纤维和胶原纤维之间间隙内的水，最终导致SCBW的粘液变性。天然金属离子螯合剂通过螯合Ca ~（2+）抑制MMP的活化，从而有效地阻止SCBW的自溶。
Live sea cucumbers (Stichopus japonicus) were stored in a solution containing oxalic acid and tea polyphenols as natural metal ion chelators. The inhibitory effects of these chelators on the autolysis phenomenon and the underlying mechanism of action were investigated for the first time by using scanning electron microscopy, differential scanning calorimetry, low-field nuclear magnetic resonance and confocal laser scanning microscopy. External stimuli cause autolysis through the release of calcium ions (Ca2+) from cells into the extracellular connective tissue, initiating activity of the matrix metalloprotease (MMP) in the sea cucumber body wall (SCBW). MMP subsequently degrades the microfibrillar networks, that support the interconnecting collagen fibres and the interfibrillar proteoglycan bridges linking the collagen fibrils, to release the water restricted within the interspaces between collagen fibres and collagen fibrils, ultimately causing mucoid degeneration of SCBW. The natural metal ion chelators significantly inhibited the activation of MMP by chelating Ca2+, consequently effectively preventing the autolysis of SCBW.