CELLS THAT EXPRESS BRAIN-DERIVED NEUROTROPHIC FACTOR MESSENGER-RNA IN THE DEVELOPING POSTNATAL RAT-BRAIN
CELLS THAT EXPRESS BRAIN-DERIVED NEUROTROPHIC FACTOR MESSENGER-RNA IN THE DEVELOPING POSTNATAL RAT-BRAIN
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DOI：
10.1111/j.1460-9568.1991.tb00854.x
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发表时间：
1991-07-01
影响因子：
3.4
通讯作者：
PERSSON, H
中科院分区：
文献类型：
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作者：
FRIEDMAN, WJ;OLSON, L;PERSSON, H
脑源性神经营养因子 (BDNF) 是相关神经营养蛋白家族的成员，其中包括神经生长因子 (NGF) 和海马源性神经营养因子/神经营养蛋白-3 (NT-3)。为了获得有关 BDNF 在出生后大脑发育过程中可能发挥的作用的信息，我们使用原位杂交检查了该营养因子的时间和空间表达。在特定的新皮质区域，在 2 周龄及之后可见表达 BDNF mRNA 的细胞。一种引人注目的特殊神经元细胞类型是顶颞叶皮层深层的倒锥体细胞群。在梨状皮层和扣带皮层中，分别在出生后第 1 天和第 1 周龄检测到 BDNF mRNA，并在个体发育期间水平不断增加。几个前脑区域，包括丘脑前室旁核、下丘脑腹内侧核以及视前区，在整个发育过程中含有中等水平的 BDNF mRNA。在几个脑干结构中短暂检测到 BDNF mRNA，特别是在黑质和脚间核中。这种营养因子在新生儿早期大脑海马中的表达相对较低，但在 2 周龄时在 CA3 和 CA4 区域以及齿状回中达到高水平。在这个早期阶段，仍处于齿状回神经发生时期，标记仅限于外层，其中包含具有更成熟外观的细胞。然而，到了 3 周龄时，标记已分布在整个颗粒细胞层。我们的结果显示，在发育中的大鼠大脑的各个区域中，BDNF mRNA 都有短暂和持续的表达，并表明在出生后大脑发育过程中，BDNF 表达存在尾侧到头侧的梯度，这可能与神经元成熟相关。
Brain-derived neurotrophic factor (BDNF) is a member of a family of related neurotrophic proteins which includes nerve growth factor (NGF) and hippocampus-derived neurotrophic factor/neurotrophin-3 (NT-3). To obtain information regarding possible roles for BDNF during postnatal brain development, we have examined the temporal and spatial expression of this trophic factor using in situ hybridization. In specific neocortical regions BDNF mRNA-expressing cells were seen at 2 weeks of age and thereafter. One particular neuronal cell type strikingly labelled was the inverted pyramidal cell population in the deep layers of parietotemporal cortex. In pyriform and cingulate cortices, BDNF mRNA was detected at postnatal day 1 and 1 week of age, respectively, with increasing levels during ontogeny. Several forebrain regions, including the thalamic anterior paraventricular nucleus, hypothalamic ventromedial nucleus as well as the preoptic area, contained moderate levels of BDNF mRNA throughout development. BDNF mRNA was detected transiently in several brainstem structures, notably in the substantia nigra and interpeduncular nucleus. Expression of this trophic factor in hippocampus was relatively low in the early neonatal brain, but attained high levels in the CA3 and CA4 regions as well as in the dentate gyrus by 2 weeks of age. At this early age, which is still during the period of neurogenesis in the dentate gyrus, labelling was restricted to the outer layer, which contained cells with a more mature appearance. However, by 3 weeks of age labelling was distributed throughout the granule cell layer. Our results show both transient and persistent expression of BDNF mRNA in various regions of the developing rat brain and suggest that there is a caudal to rostral gradient of BDNF expression during postnatal brain development, which may be correlated to neuronal maturation.