Genome-wide analysis of the laccase gene family in wheat and relationship with arbuscular mycorrhizal colonization
Genome-wide analysis of the laccase gene family in wheat and relationship with arbuscular mycorrhizal colonization
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DOI：
10.1007/s00425-022-04048-1
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发表时间：
2023-01-01
期刊：
影响因子：
4.3
通讯作者：
Tian，Hui
中科院分区：
文献类型：
--
作者：
Zhong，Xiong;Li，Mengjiao;Tian，Hui
主要结论在小麦基因组中鉴定出156个漆酶基因，分属于11个亚科，漆酶基因的自然变异显著影响小麦-丛枝菌根共生系统的发育。本研究旨在揭示LAC基因在小麦基因组中的发生、染色体空间分布、共表达和进化规律，并探讨LAC基因在丛枝菌根（AM）共生过程中的可能作用。利用生物信息学方法分析了LAC基因的基因组特征，并以289个小麦品种为材料，分析了LAC基因的多态性。在小麦基因组中共鉴定出156个LAC基因，分属于11个亚家族，LAC基因家族的扩增以片段重复为主，拉克主要位于小麦染色体的R2区。某些LACs与拟南芥或水稻中的特征LACs共线。在小麦中，许多转录因子、激酶和磷酸酶基因与LACs基因共表达，TaLACs可能是某些miRNAs的潜在靶基因。大多数TaLACs主要在植物的根和茎中表达。接种禾谷镰刀菌或AM真菌可调控TaLACs的表达。TaLACs的多态性主要通过随机漂移而不是选择积累。通过候选基因关联分析，发现TaLACs的自然变异显著影响AM真菌的根系定殖。本研究为进一步研究小麦LAC基因的生物学功能，特别是LAC基因在AM真菌共生过程中的作用提供了有用的信息。
Main conclusionWe identified 156 laccase genes belonging to 11 subfamilies in the wheat genome, and the natural variation of laccase genes significantly affected the development of wheat–arbuscular mycorrhizal symbiosis.AbstractLaccases (LACs) have a variety of functions in plant lignification, cell elongation and stress responses. This study aimed to reveal the phylogeny, chromosomal spatial distribution, coexpression and evolution ofLACgenes in the wheat genome and to investigate the possible roles ofLACgenes during arbuscular mycorrhizal (AM) symbiosis. The genomic characteristics ofLACgenes were analyzed by using bioinformatics analysis methods, and the polymorphisms ofLACgenes were analyzed by using a diverse wheat panel composed of 289 wheat cultivars. We identified 156LACgenes belonging to 11 subfamilies in the wheat genome, and segmental duplication dominated the amplification of theLACgene family in the wheat genome.LACs are dominantly located in the R2 region of wheat chromosomes. SomeLACs are collinear with the characterizedLACs inArabidopsis thalianaor rice. A number of genes encoding transcription factors, kinases, and phosphatases were coexpressed withLACgenes in wheat.TaLACs may be potential targets for some miRNAs. MostTaLACs are mainly expressed in the roots and stems of plants. The expression ofTaLACs could be regulated by the inoculation ofFusarium graminearumor AM fungi. The polymorphisms ofTaLACs mainly accumulate by random drift instead of by selection. Through candidate gene association analysis, we found that the natural variations inTaLACs significantly affected root colonization by AM fungi. The present study provides useful information for further study of the biological functions ofLACgenes in wheat, especially the roles ofLACgenes during the development of AM symbiosis.