Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis
Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis
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DOI：
10.1074/jbc.274.24.17132
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发表时间：
1999-06-11
影响因子：
4.8
通讯作者：
Han, KH
中科院分区：
文献类型：
--
作者：
Oh, SK;Kang, H;Han, KH
迄今为止报道的生化证据表明，橡胶合成发生在橡胶树胶乳中悬浮的橡胶颗粒表面。我们已经分离和表征了一个cDNA克隆，其编码紧密结合在小橡胶颗粒上的蛋白质。我们将这种蛋白命名为小橡胶颗粒蛋白（SRPP），在本研究之前，这种蛋白被认为是一种乳胶过敏原，只有其部分氨基酸序列被报道。序列分析表明，该蛋白与橡胶延伸因子和菜豆胁迫相关蛋白高度同源。Southern和北方分析表明，该蛋白质是由一个单一的基因编码，并在胶乳中高度表达。使用重组蛋白的变应原性试验证实，克隆的cDNA编码已知的24-kDa乳胶过敏原。无论是乙烯刺激还是伤害都没有改变H.巴西的。体外橡胶测定表明，该蛋白在橡胶生物合成中起着积极的作用。因此，SRPP可能是橡胶生物合成机制的一部分，如果不是橡胶聚合酶，则与橡胶延伸因子一起沿着。
Biochemical evidence reported so far suggests that rubber synthesis takes place on the surface of rubber particles suspended in the latex of Hevea brasiliensis. We have isolated and characterized a cDNA clone that encodes a protein tightly bound on a small rubber particle. We named this protein small rubber particle protein (SRPP), Prior to this study, this protein was known as a latex allergen, and only its partial amino acid sequence was reported. Sequence analysis revealed that this protein is highly homologous to the rubber elongation factor and the Phaseolus vulgaris stress-related protein. Southern and Northern analyses indicate that the protein is encoded by a single gene and highly expressed in latex. An allergenicity test using the recombinant protein confirmed that the cloned cDNA encodes the known 24-kDa latex allergen. Neither ethylene stimulation nor wounding changed the transcript level of the SRPP gene in H. brasiliensis. An in vitro rubber assay showed that the protein plays a positive role in rubber biosynthesis. Therefore, it is likely that SRPP is a part of the rubber biosynthesis machinery, if not the rubber polymerase, along with the rubber elongation factor.