New thioester substrates for fibrinoligase (coagulation factor XIIIa) and for transglutaminase. Transfer of the fluorescently labeled acyl group to amines and alcohols.
New thioester substrates for fibrinoligase (coagulation factor XIIIa) and for transglutaminase. Transfer of the fluorescently labeled acyl group to amines and alcohols.
复制标题
纤维蛋白连接酶（凝血因子 XIIIa）和转谷氨酰胺酶的新硫酯底物。
DOI：
10.1021/bi00516a006
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Lorand,L
中科院分区：
文献类型：
--
作者：
Parameswaran,KN;Lorand,L
Materials and MethodsGuinea pig liver transglutaminase was prepared by Dr. Y. S. Tong of this laboratory essentially by the procedure of Connellan et al.(1971) and was stored frozenin 50 mM Tris-HCl buffer, pH 7.5, containing 1 mM EDTA. This endo-7-glutamine: e-lysine transferase was employed for the screening of the newly synthesized fluorescent thiocholine ester substrates, using the thin-layer chromatographic analytical procedure given below.
