Nerve growth factor rescue of cisplatin neurotoxicity is mediated through the high affinity receptor: studies in PC12 cells and p75 null mouse dorsal root ganglia
Nerve growth factor rescue of cisplatin neurotoxicity is mediated through the high affinity receptor: studies in PC12 cells and p75 null mouse dorsal root ganglia
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DOI：
10.1016/s0304-3940(01)01956-5
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发表时间：
2001-07-27
影响因子：
2.5
通讯作者：
Windebank, AJ
中科院分区：
文献类型：
--
作者：
Fischer, SJ;Podratz, JL;Windebank, AJ
神经生长因子（NGF）从顺铂诱导的细胞死亡中拯救背根神经节神经元和PC 12细胞。两个模型系统被用来证明救援是通过高亲和力的NGF受体介导的。在分离自p75（-/-）和对照小鼠的背根神经节（DRG）神经元中，20 ng/ml NGF完全阻止顺铂诱导的死亡。在PC 12细胞中，我们过度表达肿瘤坏死因子和NGF受体之间的受体嵌合体。我们证明了Trk A的胞内结构域的激活负责NGF的拯救作用。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Nerve growth factor(NGF) rescues dorsal root ganglion neurons and PC12 cells from cisplatin-induced cell death. Two model systems were used to demonstrate that rescue is mediated through the high affinity NGF receptor. In dorsal root ganglion (DRG) neurons isolated from p75(-/-) and control mice, 20 ng/ml NGF completely prevented cisplatin-induced death. In PC12 cells, we overexpressed receptor chimeras between the tumor necrosis factor and NGF receptors. We demonstrated that activation of the intracellular domain of Trk A is responsible for the NGF rescue effect. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.