THE NUCLEATOR
THE NUCLEATOR
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DOI：
10.1111/j.1365-2818.1988.tb04609.x
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发表时间：
1988-07-01
影响因子：
2
通讯作者：
GUNDERSEN, HJG
中科院分区：
文献类型：
--
作者：
GUNDERSEN, HJG
一个新的，基本的体视学原理，它允许无偏估计的绝对结构量在任意形状的结构，从独立的各向同性探针上的任意点采样的观察。作为一个介绍的原则，方法（S）的描述，导致从通常的数量分布的颗粒尺寸的平均体积的无偏估计。具体地说，估计是无偏的任意形状的粒子时，采样均匀的概率使用disector或其许多修改之一。当观察仅限于切片时，当粒子与某些可识别的单位（如生物学中的真核细胞）相关联时，会出现一个特殊的情况。然后，可以对几个未知厚度的截面进行估计，或者在可验证的假设下对一个随机截面进行估计。
A new, fundamental stereological principle is described which allows unbiased estimates of absolute structural quantities in arbitrarily shaped structures to be made from observations sampled in arbitrary points on independently isotropic probes. As an introduction to the principle, method(s) are described which lead to assumption-free estimates of mean volume from the usual number-distribution of particle sizes. Specifically, the estimation is unbiased when arbitrarily shaped particles are sampled with uniform probability using the disector or one of its many modifications. A special case, of interest when observations are restricted to sections, occurs when the particles are associated with some recognizable unit, like eukaryotic cells in biology. The estimation may then be carried out on a few sections of unknown thickness, or.sbd.under verifiable assumptions.sbd.on just one random section.