Peripheral and central augmentation indexes in relation to the CYP4F2 polymorphisms in Chinese
Peripheral and central augmentation indexes in relation to the CYP4F2 polymorphisms in Chinese
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中国人与 CYP4F2 多态性相关的外周和中枢增强指数
DOI：
10.1097/hjh.0b013e328342673c
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发表时间：
2011-03
影响因子：
4.9
通讯作者：
Li Yan
中科院分区：
文献类型：
--
作者：
Zhang, Yi;Li, Fa-Hong;Wang, Ji-Guang;Fan, Hong-Qi;Hu, BangChuan;Sheng, Chang-Sheng;Li Yan
目的细胞色素(CYP)4F2亚型是肾脏20-羟基二十碳四烯酸(20-HETE)的关键代谢酶，作为一种内源性血管收缩因子，可影响周围肌肉动脉和小动脉的特性。因此，我们研究了用增强指数(AIX)测量的中国人中与动脉波反射有关的细胞色素P4F2基因多态性。方法采用SPhygmoCor对1421例静宁人群进行动脉测量，并采用聚合酶链式反应-限制性片段长度多态方法对3个CYP4F2基因(V433M、rs3093089和rs3093098)进行基因分型。对924名从工作场所招募的中国人进行了V433M多态的复制研究。采用酶联免疫吸附试验(EL ISA)测定了318例静宁人群的尿液20-HETE浓度。结果尽管所有静宁受试者的遗传关联均不显著(P≥0.12)，但V433M基因多态与性别和脉率之间存在显著交互作用(Pint≤0.0 2)，且与外周和中枢AIX相关。与V433V纯合子相比，M433等位基因携带者的外周血型(+5.0%，P=0.0002)和中央型AIX(+3.2%，P=0.001)显著增加。脉率最高三分位数(≥76次/分)的490名受试者的对应值分别为+2.7%(P=0.0 4)和+1.9%(P=0.0 4)；M433M(2.06 ng/ml)和V433M(1.13 ng/ml)个体的尿20-HETE浓度显著高于V433V纯合子(0.98 ng/ml)(P=0.002)。结论在中国男性人群中，CYP4F2基因V433M多态与动脉波反射的大小或脉率较快的个体相关。
Objective Cytochrome (CYP) 4F2 isoform is a key metabolizing enzyme for the renal 20-hydroxyeicosatetraenoic acid (20-HETE), which, as an endogenous vasoconstrictor, may influence properties of the peripheral muscular arteries and arterioles. We, therefore, investigated the CYP4F2 polymorphisms in relation to arterial wave reflections, as measured by augmentation indexes (AIx) in Chinese. Methods We performed arterial measurements by SphygmoCor and genotyped three CYP4F2 polymorphisms (V433M, rs3093089, and rs3093098) by PCR-restriction fragment length polymorphism in 1421 participants enrolled in the JingNing Population study. A replication study for the V433M polymorphism was performed in 924 Chinese recruited from a workplace setting. Urinary 20-HETE concentration was determined by ELISA in a randomly selected subsample of 318 JingNing individuals. Results In spite of the fact that genetic associations were not significant (P ≥ 0.12) in all JingNing participants, there was significant (Pint ≤ 0.02) interaction of the V433M polymorphism with sex and pulse rate in relation to peripheral and central AIx. M433 allele carriers, compared with V433V homozygotes, had significantly greater peripheral (+5.0%, P = 0.0002) and central AIx (+3.2%, P = 0.001) in 693 men. The corresponding values were +2.7% (P = 0.04) and +1.9% (P = 0.04) in 490 individuals of the top tertile of pulse rate (≥76 beats/min), and were +4.0% (P = 0.02) and +3.3% (P = 0.02) in 315 replication participants with a pulse rate at least 76 beats/min. Urinary 20-HETE concentration was significantly higher (P = 0.002) in M433M (2.06 ng/ml) and V433M (1.13 ng/ml) individuals than in V433V homozygotes (0.98 ng/ml). Conclusion The CYP4F2 V433M polymorphism is associated with the size of arterial wave reflections in male Chinese, or individuals with a faster pulse rate.
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影响因子：
2.6
作者：
Ding H;Cui G;Zhang L;Xu Y;Bao X;Tu Y;Wu B;Wang Q;Hui R;Wang W;Dackor RT;Kissling GE;Zeldin DC;Wang DW
通讯作者：
Wang DW
影响因子：
39.3
作者：
Vlachopoulos, Charalambos;Aznaouridis, Konstantinos;Stefanadis, Christodoulos
通讯作者：
Stefanadis, Christodoulos
影响因子：
8.3
作者：
Singh, Harpreet;Cheng, Jennifer;Schwartzman, Michal Laniado
通讯作者：
Schwartzman, Michal Laniado
功能性细胞色素 P4504F2 单倍型与尿 20-HETE 和高血压的关联
影响因子：
13.6
作者：
Liu, Hong;Zhao, Yanyan;Lu, Jingyu
通讯作者：
Lu, Jingyu
DOI：
10.1073/pnas.081627898
发表时间：
2001-04-24
影响因子：
11.1
作者：
Holla, VR;Adas, F;Capdevila, JH
通讯作者：
Capdevila, JH