Vasoactive intestinal polypeptide immunoreactive neurons in the primary visual cortex of the cat.
Vasoactive intestinal polypeptide immunoreactive neurons in the primary visual cortex of the cat.
复制标题
猫初级视觉皮层中的血管活性肠多肽免疫反应神经元。
DOI：
10.1007/bf02456695
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Karamanlidis,AN
中科院分区：
文献类型：
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作者：
Peters,A;Meinecke,DL;Karamanlidis,AN
当猫视觉皮层（17区）与血管活性肠多肽（VIP）抗体反应时，多种神经元类型被标记。许多被标记的神经元在形式上是双极的，最常见的是在第II层和第III层，尽管在第v层也遇到了大量的双极神经元。这些是最常见的在第四层，有各种各样的形状。在第一层中，标记细胞分为水平双极细胞、水平沥青细胞和多极神经元三种，而在第六层中，少数vip阳性神经元为水平双极细胞。这表明17区所有vip标记的神经元在形态上都是非锥体的，这已被电子显微镜证实。在这些制备中，轴突终末也被标记，在光镜下可以看到这些终末出现在神经细胞内和一些神经元的细胞体周围，特别是在第II层和第III层的神经元。电子显微镜显示，所有被标记的轴突末端形成对称的突触，而那些在neuropil突触与光滑的树突轴。这些轴突突触约占由标记轴突末端形成的突触的90%。其余被标记的轴突末端与锥体神经元的细胞体发生突触。这些结果与先前通过检查大鼠视觉皮层中标记有VIP抗体的神经元元素所获得的结果相似。
When cat visual cortex (area 17) is reacted with an antibody to vasoactive intestinal polypeptide (VIP) a variety of neuronal types is labelled. Many of the labelled neurons are bipolar in form and are most common in layers II and III, although significant numbers of bipolar neurons are also encountered in layer V. Multipolar cells are also labelled. These are most frequent in layer IV and have a variety of shapes. In layer I, the labelled cells are of three varieties, i.e. horizontal bipolar cells, horizontal bitufted cells and multipolar neurons, while in layer VI the few VIP-positive neurons are horizontal bipolar cells. This suggests that all of the VIP-labelled neurons in cat area 17 are non-pyramidal in form, and this has been confirmed by electron microscopy.In these preparations, axon terminals are also labelled and under the light microscope it can be seen that these terminals occur both within the neuropil and around the cell bodies of some neurons, particularly neurons in layers II and III. Electron microscopy has shown that all of the labelled axon terminals form symmetric synapses and that those in the neuropil synapse with the shafts of smooth dendrites. These axodendritic synapses account for about 90% of the synapses formed by the labelled axon terminals. The remainder of the labelled axon terminals synapse with the cell bodies of pyramidal neurons.Parallels are drawn between these results and those previously obtained by examining those neuronal elements labelled with VIP antibodies in rat visual cortex.