DEVELOPMENT AND CLINICAL APPLICATION OF A NEW METHOD FOR RADIOIMMUNOASSAY OF ARGININE VASOPRESSIN IN HUMAN PLASMA
DEVELOPMENT AND CLINICAL APPLICATION OF A NEW METHOD FOR RADIOIMMUNOASSAY OF ARGININE VASOPRESSIN IN HUMAN PLASMA
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DOI：
10.1172/jci107423
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发表时间：
1973-01-01
影响因子：
15.9
通讯作者：
SINHA, T
中科院分区：
文献类型：
--
作者：
ROBERTSON, GL;MAHR, EA;SINHA, T
发展了一种放射免疫分析方法，可以可靠地测量血浆精氨酸加压素(AVP)的浓度，浓度低至0.5pg/ml，样品体积为1ml或更少。与血浆蛋白相关的非激素免疫反应性在检测前通过丙酮沉淀被消除，留下一个在免疫学和层析上与标准AVP无法区分的成分。血浆的储存会导致AVP浓度的下降，因此必须仔细调节。用我们的方法得到的血浆AVP值接近预期水平，并根据生理预期而变化。卧位正常人血浆血管紧张素转运蛋白的范围为(Mean±SD)5.4±3.4pg/ml~(1.4±0.8pg/ml)，与血浆渗透压(r=0.52p<；0.001)和尿渗透压(r=0.77p<；0.001)均呈显著正相关。限制液体后，肾源性尿崩症和原发性多饮患者的血浆AVP与血浆渗透压均正常，而所有垂体型尿崩症患者的血浆AVP均明显低于正常。生理剂量的垂体后叶提取物使血浆加压素浓度呈剂量依赖性升高，随后以预期的速度下降(tç=22.5±4min)。我们的结论是，如果使用得当，我们的放射免疫分析方法为评估人类的AVP功能提供了一种有用的方法。
A radioimmunoassay has been developed that permits reliable measurements of plasma arginine vasopressin (AVP) at concentrations as low as 0.5 pg/ml in sample volumes of 1 ml or less. Nonhormonal immunoreactivity associated with the plasma proteins is eliminated by acetone precipitation before assay, leaving unaltered a component that is immunologically and chromatographically indistinguishable from standard AVP. Storage of plasma results in a decline in AVP concentration and, thus, must be carefully regulated. The plasma AVP values obtained by our method approximate the anticipated levels and vary in accordance with physiologic expections. In recumbent normal subjects, plasma AVP ranged from (mean ±SD) 5.4±3.4 pg/ml after fluid deprivation to 1.4±0.8 pg/ml after water loading, and correlated significantly with both plasma osmolality (r=0.52;P<0.001) and urine osmolality (r=0.77;P<0.001). After fluid restriction, plasma AVP was uniformly normal relative to plasma osmolality in patients with nephrogenic diabetes insipidus and primary polydipsia but was distinctly subnormal in all patients with pituitary diabetes insipidus. The infusion of physiologic amounts of posterior pituitary extract caused a dose-related rise in plasma vasopressin that afterwards declined at the expected rate (t½=22.5±4 min). We conclude that, when used appropriately, our radioimmunoassay method provides a useful way of assessing AVP function in man.