Laser desorption postionization for imaging MS of biological material.
Laser desorption postionization for imaging MS of biological material.
复制标题
DOI：
10.1002/jms.1716
复制
发表时间：
2010-02
影响因子：
2.3
通讯作者：
Hanley, Luke
中科院分区：
文献类型：
--
作者：
Akhmetov, Artem;Moore, Jerry F.;Gasper, Gerald L.;Koin, Peter J.;Hanley, Luke
真空紫外单光子电离（VUV SPI）是一种软电离技术，有可能解决许多MALDI成像MS的局限性。激光解吸定位（LDPI）采用VUV SPI定位，并在实验上类似于MALDI仪器，增加了脉冲VUV光源。本文综述了在LDPI-MS在过去十年中的进展，重点是成像MS的细菌生物膜，分析物的高盐环境，使他们特别耐成像MALDI-MS。本文首先考虑VUV SPI的基本方面，包括电离机制，横截面，电离，解离的量子产率，和潜在的质量限制。然后沿着新构建的具有成像功能的LDPI-MS仪器描述了最常见的脉冲VUV辐射源。接下来，通过LDPI-MS使用来自分子氟准分子激光器的7.87 eV（157.6 nm）VUV光子，随后使用芳香族标签检测生物膜内的选定物种，来证明完整生物膜内的小分子的检测和成像。最后一节考虑了未来的前景成像完整的生物样品的低密度聚阴离子质谱。
Vacuum ultraviolet single photon ionization (VUV SPI) is a soft ionization technique that has the potential to address many of the limitations of MALDI for imaging MS. Laser desorption postionization (LDPI) employs VUV SPI for postionization and is experimentally analogous to a MALDI instrument with the addition of a pulsed VUV light source. This review discusses progress in LDPI-MS over the last decade, with an emphasis on imaging MS of bacterial biofilms, analytes whose high salt environment make them particularly resistant to imaging by MALDI-MS. This review first considers fundamental aspects of VUV SPI including ionization mechanisms, cross sections, quantum yields of ionization, dissociation, and potential mass limits. The most common sources of pulsed VUV radiation are then described along with a newly constructed LDPI-MS instrument with imaging capabilities. Next, the detection and imaging of small molecules within intact biofilms is demonstrated by LDPI-MS using 7.87 eV (157.6 nm) VUV photons from a molecular fluorine excimer laser, followed by the use of aromatic tags for detection of selected species within the biofilm. The final section considers the future prospects for imaging intact biological samples by LDPI-MS.
登录
查看更多内容
影响因子：
3.8
作者：
Heinbuch, S;Grisham, M;Rocca, JJ
通讯作者：
Rocca, JJ
影响因子：
1.8
作者：
Hanna, S. J.;Campuzano-Jost, P.;Bertram, A. K.
通讯作者：
Bertram, A. K.
影响因子：
7.4
作者：
Edirisinghe, Praneeth D.;Moore, Jerry F.;Hanley, Luke
通讯作者：
Hanley, Luke
影响因子：
7.4
作者：
Hanley, Luke;Zimmermann, Ralf
通讯作者：
Zimmermann, Ralf
影响因子：
6.7
作者：
Hanley, L.;Edirisinghe, P. D.;Moore, J. F.
通讯作者：
Moore, J. F.