Rapid and Nondestructive Evaluation of Platelet Function in Whole Blood by Microfluidic Deterministic Cytometry
Rapid and Nondestructive Evaluation of Platelet Function in Whole Blood by Microfluidic Deterministic Cytometry
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DOI：
10.1021/acs.analchem.3c03400
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发表时间：
2023-12-26
影响因子：
7.4
通讯作者：
Liu，Lihong
中科院分区：
文献类型：
--
作者：
Chen，Yue;Deng，Jieqi;Liu，Lihong
血小板大小是血小板功能的决定因素。这里，开发了一种新型微流控确定性细胞仪，其填充有S形微柱（S-MDC），可快速、灵敏地测定血小板的表观尺寸（Dapp），用于评估血小板功能。在模拟生理条件的剪切速率 (0.4 mm/s) 下，S-MDC 在 2 分钟内快速、无标记地测量稀释全血中的血小板 Dapp。血小板在整个S-MDC前后血小板上CD62p水平几乎没有变化，表明血小板功能是非破坏性的。值得注意的是，S-MDC添加凝血酶前后测定的人血小板Dapp与流式细胞术测定的血小板表面CD62p水平高度一致(r=0.819)，表明人血小板Dapp可用于评估血小板活化状态。此外，大鼠血小板Dapp结果与心肌缺血大鼠抗血小板药物治疗前后心肌损伤情况一致，提示大鼠血小板Dapp可用于反映心肌损伤的体内转归。这些发现表明，除了监测抗血小板药物外，S-MDC 也是一种很有前景的出血性疾病筛查测试技术。
Platelet size is a determinant of platelet function. Here, a new microfluidic deterministic cytometry packed withS-shaped micropillars (S-MDC) was developed to rapidly and sensitively determine the apparent size (Dapp) of platelets, which was used to evaluate platelet function. The plateletDappin the diluted whole blood was rapidly and label-freely measured byS-MDC within 2 min under shear rates (0.4 mm/s) that mimicked physiological conditions. The level of CD62p on platelets scarcely changed before and after platelets went through the wholeS-MDC, indicating that the platelet function was nondestructive. Notably, the human plateletDappdetermined before and after thrombin addition byS-MDC was highly coincident with the levels of CD62p on the platelet surface by flow cytometry (r= 0.819), revealing that the human plateletDappwas available to assess the platelet activation state. In addition, the results of the rat plateletDappwere consistent with myocardial injury of rats with myocardial ischemia before and after treatment with antiplatelet agents, suggesting that rat plateletDappcan be used to reflect myocardial injuryin vivooutcomes. These findings reveal thatS-MDC is a promising technique for screening tests for a bleeding disorder, in addition to monitoring antiplatelet drugs.