Effect of low-concentration rhamnolipid on adsorption of Pseudomonas aeruginosa ATCC 9027 on hydrophilic and hydrophobic surfaces
Effect of low-concentration rhamnolipid on adsorption of Pseudomonas aeruginosa ATCC 9027 on hydrophilic and hydrophobic surfaces
复制标题
低浓度鼠李糖脂对亲水和疏水表面铜绿假单胞菌 ATCC 9027 吸附的影响
DOI：
10.1016/j.jhazmat.2014.11.050
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发表时间：
2015
影响因子：
13.6
通讯作者：
He Yibin
中科院分区：
文献类型：
--
作者：
Zhong Hua;Jiang Yongbing;Zeng Guangming;Liu Zhifeng;Liu Liuxia;Liu Yang;Yang Xin;Lai Mingyong;He Yibin
采用批量吸附实验研究了低浓度单hamorl （monoRL）对生长在葡萄糖或十六烷上的铜绿假单胞菌atcc 9027在疏水或亲水表面玻璃珠上的吸附作用。结果表明，细胞在两种玻璃微珠上的吸附等温线更符合Freundlich方程，而不是Langmuir方程。十六烷生长的细胞对疏水表面的玻璃珠的吸附的Freundlich方程的kf显著高于十六烷生长的细胞对亲水表面的玻璃珠的吸附，也高于葡萄糖生长的细胞对亲水或疏水表面的玻璃珠的吸附。随着单orl浓度的增加，其含量逐渐降低。两组细胞的zeta电位均随着单orl浓度的增加而趋于稳定。十六烷培养的细胞表面疏水性显著高于葡萄糖培养的细胞，并随着单orl浓度的增加而降低。结果表明，疏水相互作用对细菌细胞对表面的吸附具有重要作用，单orl通过影响细胞表面疏水性来减少细菌的吸附。
The effects of low-concentration monorhamnolipid (monoRL) on the adsorption ofPseudomonas aeruginosaATCC 9027 grown on glucose or hexadecane to glass beads with hydrophobic or hydrophilic surfaces was investigated using batch adsorption experiments. Results showed that adsorption isotherms of the cells on both types of glass beads fitted the Freundlich equation better than the Langmuir equation. TheKfof the Freundlich equation for adsorption of hexadecane-grown cell to glass beads with hydrophobic surface was remarkably higher than that for adsorption of hexadecane-grown cell to glass beads with hydrophilic surface, or glucose-grown cell to glass beads with either hydrophilic or hydrophobic surface. Furthermore, it decreased with the increasing monoRL concentration. For both groups of cells, the zeta potential was close to each other and stable with the increase of monoRL concentration. The surface hydrophobicity of hexadecane-grown cells, however, was significantly higher than that of the glucose-grown cells and it decreased with the increase of monoRL concentration. The results indicate the importance of hydrophobic interaction on adsorption of bacterial cells to surfaces and monoRL plays a role in reducing the bacterial adsorption by affecting cell surface hydrophobicity.