Correlation of electrophysiology, neurochemistry and axonal projections of guinea-pig sphincter of Oddi neurones.
Correlation of electrophysiology, neurochemistry and axonal projections of guinea-pig sphincter of Oddi neurones.
复制标题
豚鼠 Oddi 神经元括约肌的电生理学、神经化学和轴突投影的相关性。
DOI：
10.1046/j.1365-2982.1998.00101.x
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Mawe,GM
中科院分区：
文献类型：
--
作者：
Hillsley,K;Mawe,GM
Oddi括约肌(Sphincter of Oddi，SO)神经节根据其电或神经化学性质由两种主要类型的神经元组成。这项研究调查了这些细胞的电和神经化学性质之间是否存在任何相关性。用神经生物素标记后，对神经元进行β-烟酰胺腺嘌呤二核苷酸磷酸黄递酶(NADPH-DA)染色和胆碱乙酰转移酶免疫反应，以确定神经元是硝能的还是胆碱能的。以这种方式分析了总共119个细胞；45%的细胞是张力细胞，37%的细胞是时相细胞。张力细胞(58.1%，18/31)和时相细胞(60%，21/35)胆碱乙酰转移酶(ChAT)阳性。34个时相细胞中3个为NADPH-DA阳性，11/33个张力细胞为NADPH-DA阳性。未观察到ChAT免疫反应和NADPH-DA反应出现在同一神经元中。Calretinin免疫反应存在于紧张型和相型神经元的亚群中。轴突投射的方向与细胞的电生理或神经化学特性之间没有相关性。这些结果表明，SO神经元的电特性和神经化学含量之间缺乏相关性。对这些发现的各种解释进行了讨论。
Sphincter of Oddi (SO) ganglia are comprised of two main types of neurones based either on their electrical or neurochemical properties. This study investigated whether any correlation exists between the electrical and neurochemical properties of these cells. SO neurones were characterized electrically as either Tonic or Phasic cells, labelled with neurobiotin, fixed, and processed for β‐nicotinamide adenine dinucleotide phosphate diaphorase (NADPH‐DA) staining and choline acetyltransferase immunoreactivity to identify whether electrically characterized neurones were nitrergic or cholinergic. A total of 119 cells were analysed in this manner; 45% of cells were Tonic and 37% were Phasic. An equivalent number of Tonic (58.1%, 18/31) and Phasic cells (60%, 21/35) were choline acetyltransferase (ChAT) positive. Three of 34 Phasic cells were NADPH‐DA positive, whereas 11/33 Tonic cells were NADPH‐DA positive. In none of the preparations was ChAT immunoreactivity and NADPH‐DA reactivity ever observed in the same neurone. Calretinin immunoreactivity was present in a subpopulation of both Tonic and Phasic neurones. No correlation was observed between the direction of axon projections and the electrophysiological or neurochemical properties of the cell. These results suggest that there is a lack of correlation between the electrical properties and the neurochemical content of SO neurones. Various explanations for these findings are discussed.