Energy generation from the CO oxidation-hydrogen production pathway in Rubrivivax gelatinosus
Energy generation from the CO oxidation-hydrogen production pathway in Rubrivivax gelatinosus
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DOI：
10.1128/aem.71.6.2870-2874.2005
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发表时间：
2005-06-01
影响因子：
4.4
通讯作者：
Vanzin, G
中科院分区：
文献类型：
--
作者：
Maness, PC;Huang, J;Vanzin, G
当在 CO 气体存在下孵育时，Rubrivivax gelatinosus CBS 会根据化学计量 CO + H2O -> CO2 + H-2 诱导 CO 氧化-H 生成途径。一旦被诱导，这条通路在光明和黑暗中都同样顺利地进行。当没有光照时，CO 可以作为唯一的碳源，支持细胞厌氧生长，细胞倍增时间接近 2 天。这一观察结果表明 CO 氧化反应产生能量。事实上，在向培养物气相中添加 CO 后，在黑暗中检测到了新的 ATP 合成，这与接受惰性气体（如氩气）的对照情况形成鲜明对比。当在电子传递解偶联剂羰基氰间氯苯腙 (CCCP) 存在下测定 CO 转化为 H-2 的活性时，CO 氧化产生 H 的速率比对照提高了近 40%。添加 ATP 合酶抑制剂 NN'-二环己基碳二亚胺 (DCCD) 后，我们观察到 CO 氧化产生的 H-2 受到抑制，而添加 CCCP 后可以逆转这种抑制。总的来说，这些数据强烈表明，CO 转化为 H 的反应产生了由跨膜质子梯度驱动的 ATP，但该反应的详细机制尚不清楚。这些发现鼓励开展更多研究，旨在从含二氧化碳的气流中长期生产氢气。
When incubated in the presence of CO gas, Rubrivivax gelatinosus CBS induces a CO oxidation-H, production pathway according to the stoichiometry CO + H2O -> CO2 + H-2. Once induced, this pathway proceeds equally well in both light and darkness. When light is not present, CO can serve as the sole carbon source, supporting cell growth anaerobically with a cell doubling time of nearly 2 days. This observation suggests that the CO oxidation reaction yields energy. Indeed, new ATP synthesis was detected in darkness following CO additions to the gas phase of the culture, in contrast to the case for a control that received an inert gas such as argon. When the CO-to-H-2 activity was determined in the presence of the electron transport uncoupler carbonylcyanide m-chlorophenylhydrazone (CCCP), the rate of H, production from CO oxidation was enhanced nearly 40 % compared to that of the control. Upon the addition of the ATP synthase inhibitor NN'-dicyclohexylcarbodiimide (DCCD), we observed an inhibition of H-2 production from CO oxidation which could be reversed upon the addition of CCCP. Collectively, these data strongly suggest that the CO-to-H, reaction yields ATP driven by a transmembrane proton gradient, but the detailed mechanism of this reaction is not yet known. These findings encourage additional research aimed at long-term H2 production from gas streams containing CO.