Circular Permutation of a WW Domain: Folding Still Occurs after Excising the Turn of the Folding-Nucleating Hairpin
Circular Permutation of a WW Domain: Folding Still Occurs after Excising the Turn of the Folding-Nucleating Hairpin
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DOI：
10.1021/ja410824x
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发表时间：
2014-01-15
影响因子：
15
通讯作者：
Andersen, Niels H.
中科院分区：
文献类型：
--
作者：
Kier, Brandon L.;Anderson, Jordan M.;Andersen, Niels H.
一个超稳定的Pin1 WW结构域通过去除褶皱成核弯得到了循环排列；它仍然可以折叠形成天然的三股薄片和疏水核心特征。正常和环状排列序列的多探针折叠动力学研究，以及它们的组成发夹片段和相当长度的β -链-环-链模型。指示。所有拓扑的双态折叠。n端发夹形成是野生型序列的褶皱成核事件；较慢折叠的圆形排列体具有更分散的折叠过渡态。
A hyperstable Pin1 WW domain has been circularly permuted via excision of the fold-nucleating turn; it still folds to form the native three-strand sheet and hydrophobic core features. Multiprobe folding dynamics studies of the normal and circularly permuted sequences, as well as their constituent hairpin fragments and comparable-length beta-strand-loop-beta-strand models,. indicate. 2-state folding for all topologies. N-terminal hairpin formation is the fold nucleating event for the wild-type sequence; the slower folding circular permutant has a more distributed folding transition state.