Ratiometric upconversion nanothermometry with dual emission at the same wavelength decoded via a time-resolved technique
Ratiometric upconversion nanothermometry with dual emission at the same wavelength decoded via a time-resolved technique
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DOI：
10.1038/s41467-019-13796-w
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发表时间：
2020-01-07
影响因子：
16.6
通讯作者：
Li, Fuyou
中科院分区：
文献类型：
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作者：
Qiu, Xiaochen;Zhou, Qianwen;Li, Fuyou
微环境的体内温度监测在生物学和纳米医学研究中具有重要意义。发光纳米测温法是一种无创的、高灵敏度、高响应速度的生物体内温度检测方法。然而，复杂组织中的吸收和散射限制了信号穿透深度，并且由于体内不同位置的变化而导致误差。为了最大限度地减少这些误差和监测体内温度，在本工作中，我们提供了一种策略，以制造一个相同波长的双发射比上转换发光纳米温度计的基础上的混合结构组成的上转换发光PbS量子点和Tm掺杂的上转换纳米粒子。通过时间分辨技术对由工作在相同波长但不同发光寿命的两个上转换发射组成的比率信号进行解码。该纳米温度计提高了温度监测能力，在体内的热分辨率和灵敏度分别接近0.5K和5.6%K-1。
The in vivo temperature monitoring of a microenvironment is significant in biology and nanomedicine research. Luminescent nanothermometry provides a noninvasive method of detecting the temperature in vivo with high sensitivity and high response speed. However, absorption and scattering in complex tissues limit the signal penetration depth and cause errors due to variation at different locations in vivo. In order to minimize these errors and monitor temperature in vivo, in the present work, we provided a strategy to fabricate a same-wavelength dual emission ratiometric upconversion luminescence nanothermometer based on a hybrid structure composed of upconversion emissive PbS quantum dots and Tm-doped upconversion nanoparticles. The ratiometric signal composed of two upconversion emissions working at the same wavelength, but different luminescent lifetimes, were decoded via a time-resolved technique. This nanothermometer improved the temperature monitoring ability and a thermal resolution and sensitivity of similar to 0.5K and similar to 5.6%K-1 were obtained in vivo, respectively.