CELLULAR-BINDING MECHANISM ON RAT MACROPHAGES FOR SIALYLATED GLYCOCONJUGATES, INHIBITED BY THE MONOCLONAL-ANTIBODY ED3
CELLULAR-BINDING MECHANISM ON RAT MACROPHAGES FOR SIALYLATED GLYCOCONJUGATES, INHIBITED BY THE MONOCLONAL-ANTIBODY ED3
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DOI：
10.1002/jlb.49.5.434
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发表时间：
1991-05-01
影响因子：
5.5
通讯作者：
DIJKSTRA, CD
中科院分区：
文献类型：
--
作者：
DAMOISEAUX, JGMC;DOPP, EA;DIJKSTRA, CD
单抗ED3识别一个亚群的大鼠巨噬细胞，具有高度限制的组织分布。ED3抗原和绵羊红细胞受体(SER)在小鼠体内的组织分布和体外表达非常相似。这种受体具有几乎相同的结合特性，尽管组织分布不同，与唾液酸结合受体(SAR)在大鼠体内结合神经节苷脂包裹的红细胞。在这项研究中，我们总结了有关唾液酸结合受体(SER和SAR)的现有文献。此外，我们通过抑制红细胞与单抗ED3以及新开发的等价物ED16和ED17的结合研究，确定ED3为SER。我们还表明，光胰酶处理肺泡巨噬细胞，表达SAR，导致SER样活性。这种获得的SER样活性不能被单抗ED3阻断，表明SER和SAR是不同的受体。似乎大鼠巨噬细胞可以表达两种唾液酸糖结合物受体，一种是高亲和力受体SER，可被单抗ED3识别，另一种是低亲和力受体SAR，不被单抗ED3识别。
The mAb ED3 recognizes a subpopulation of rat macrophages, with a highly restricted tissue distribution. The tissue distribution as well as the in vitro expression of the ED3 antigen and of the sheep erythrocyte receptor (SER), binding unopsonized erythrocytes in the mouse, are very similar. This receptor has almost the same binding characteristics, although a different tissue distribution, as the sialic acid binding receptor (SAR), binding ganglioside-coated erythrocytes in the rat. In this study we summarize the available literature concerning these sialic acid binding receptors (SER and SAR). Furthermore we have identified ED3 as SER by inhibition studies of erythrocyte binding with mAb ED3, as well as by the newly developed equivalents ED16 and ED17. We also show that light trypsin treatment of alveolar macrophages, expressing SAR, results in SER-like activity. This obtained SER-like activity could not be blocked by the mAb ED3, indicating that SER and SAR are different receptors. It appears that rat macrophages can express two receptors for sialylated glycoconjugates, a high-affinity receptor SER, recognized by mAb ED3, and a low-affinity receptor SAR, not recognized by mAb ED3.