Expression analysis of genes putatively involved in chicken gonadal development
Expression analysis of genes putatively involved in chicken gonadal development
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推测参与鸡性腺发育的基因的表达分析
DOI：
10.1556/abiol.58.2007.2.3
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发表时间：
2007-06-01
影响因子：
--
通讯作者：
Gong, Y.
中科院分区：
文献类型：
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作者：
Feng, Y.;Zhang, S.;Gong, Y.
在哺乳动物中，睾丸发育是由性别决定基因SRY的表达启动的，而在鸟类中性别决定的遗传触发因素仍然未知。在本研究中，涉及脊椎动物性别决定和分化的7个基因的表达在鸡胚胎性腺从第4天至第12天的孵化使用逆转录和聚合酶链反应（RT-PCR）进行了研究。结果表明，cLhx 9、cGATA 4、cVnn 1、cPpt 1、cBrd 3基因在鸡性腺发育过程中的转录水平具有性别差异，而cEki 2、cFog 2基因在两性中的表达水平相近。哺乳动物和鸡性别决定通路的比较研究结果表明，脊椎动物性别决定通路既有保守的成分，也有差异的成分：cGATA 4/cFog 2和cVnn 1的表达谱与哺乳动物相似，而其他基因的表达谱则存在一定差异。根据这些基因的表达谱分析了它们在鸡性腺发育中的可能功能。
In mammals, testis development is initiated by the expression of the sex-determining gene, SRY whereas the genetic trigger for sex determination in birds remains unknown. In the present study, the expression of seven genes implicated in vertebrate sex determination and differentiation were studied in chicken embryonic gonads from day 4 to day 12 of incubation using reverse transcription and the polymerase chain reaction (RT-PCR). Results showed transcription of cLhx9, cGATA4, cVnn1, cPpt1, cBrd3 were sexually dimorphic during chicken gonadal development, whereas cEki2, cFog2 were expressed at similar levels in both sexes. Results of comparative studies between mammals and chickens show that vertebrate sex-determining pathways comprise both conserved and divergent elements: expression profiles of cGATA4/cFog2 and cVnn1 are similar to those in mammals, while others appear some differences. Possible functions of these genes on chicken gonadal development were analyzed based on their expression profiles.