Marinifilum fragile gen. nov., sp nov., isolated from tidal flat sediment
Marinifilum fragile gen. nov., sp nov., isolated from tidal flat sediment
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DOI：
10.1099/ijs.0.009027-0
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发表时间：
2009-09-01
影响因子：
2.8
通讯作者：
Chun, Jongsik
中科院分区：
文献类型：
--
作者：
Na, Hyunsoo;Kim, Seil;Chun, Jongsik
从韩国潮滩沉积物中分离出一种兼性厌氧、中度嗜盐、革兰氏阴性丝状细菌JC2469(T)。基于16S rRNA基因序列的系统发育分析表明，该分离物属于拟杆菌门，其最近的分类亲缘是Cytophaga fermentans NCIMB 2218(T)，序列相似度为89.6%。细胞在指数生长时期呈细丝状，在固定时期呈条状。在老化培养中也观察到球形细胞。菌株JC2469(T)以iso-C-15:0（56.2%）和MK-7为主要脂肪酸和呼吸醌。根据多相研究的证据，该分离物与其他属有很大差异。本研究的系统发育和生理资料有力地表明，该分离物代表一个新属和新种，并建议将其命名为Marinifilum fragile gen. nov., sp. nov.。易碎海螺的型应变为JC2469(T) (=IMSNU 14138(T) =KCTC 22488（T) =JCM 15579(T)）。
A facultatively anaerobic, moderately halophilic, Gram-negative, filamentous bacterium, designated JC2469(T), was isolated from tidal flat sediment in Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the phylum Bacteroidetes and its closest taxonomic relative was Cytophaga fermentans NCIMB 2218(T) (89.6% sequence similarity). Cells appeared as filaments during exponential growth, but became fragmented to rods at stationary phase. Spherical cells were also observed in aged cultures. Strain JC2469(T) contained iso-C-15:0 (56.2%) and MK-7 as the predominant fatty acid and respiratory quinone, respectively. On the basis of evidence from this polyphasic study, the isolate showed substantial differences from other genera. The phylogenetic and physiological data of the present study strongly suggest that the isolate represents a novel genus and species, for which the name Marinifilum fragile gen. nov., sp. nov. is proposed. The type strain of Marinifilum fragile is JC2469(T) (=IMSNU 14138(T) =KCTC 22488(T) =JCM 15579(T)).