Immunity induced by vaccination with recombinant influenza B virus neuraminidase protein breaks viral transmission chains in guinea pigs in an exposure intensity-dependent manner.
Immunity induced by vaccination with recombinant influenza B virus neuraminidase protein breaks viral transmission chains in guinea pigs in an exposure intensity-dependent manner.
复制标题
重组乙型流感病毒神经氨酸酶蛋白疫苗接种诱导的免疫会以暴露强度依赖性方式破坏豚鼠体内的病毒传播链。
DOI：
10.1128/jvi.01057-23
复制
发表时间：
2023
影响因子：
5.4
通讯作者：
Krammer,Florian
中科院分区：
文献类型：
--
作者：
McMahon,Meagan;Tan,Jessica;O'Dell,George;KirkpatrickRoubidoux,Ericka;Strohmeier,Shirin;Krammer,Florian
黏膜疫苗和阻断病原体传播的疫苗在疫苗开发中被低估了。然而，严重急性呼吸综合征冠状病毒2(SARS-CoV-2)的大流行表明，阻断病毒传播是有效疫苗的重要属性。在这里，我们研究了重组流感病毒神经氨酸酶(NA)疫苗是否可以在豚鼠模型中防止B型流感病毒的继续传播。我们测试了四种不同的情景，在这些情景中，顺序传播被研究在三到四只豚鼠的链上。被测试的变量包括初始供体豚鼠的低和高病毒接种量(104vs105个空斑形成单位)以及暴露/共住时间的变化(1dvs6d)。在四种情况中，有三种情况--低接种量-长暴露、低接种量-短暴露和高接种量-短暴露-传播链被有效地阻断。基于这一数据，我们认为鼻内重组NA疫苗可以有效地遏制流感病毒在流感流行期间在人群中的传播。严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)和流感病毒迫切需要能够减缓呼吸道病毒在人群中传播的进口疫苗。在这里，我们描述了基于神经氨酸酶的重组流感病毒疫苗如何以暴露强度为基础的方式减少在接种疫苗的豚鼠中的传播。
Mucosal vaccines and vaccines that block pathogen transmission are under-appreciated in vaccine development. However, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has shown that blocking viral transmission is an important attribute of efficient vaccines. Here, we investigated if recombinant influenza virus neuraminidase (NA) vaccines delivered at a mucosal site could protect from onward transmission of influenza B viruses in the guinea pig model. We tested four different scenarios in which sequential transmission was investigated in chains of three to four guinea pigs. The variables tested included a low and a high viral inoculum (104vs 105plaque-forming units) in the initial donor guinea pig and variation of exposure/cohousing time (1 day vs 6 days). In three out of four scenarios—low inoculum-long exposure, low inoculum-short exposure, and high inoculum-short exposure—transmission chains were efficiently blocked. Based on this data, we believe an intranasal recombinant NA vaccine could be used to efficiently curtail influenza virus spread in the human population during influenza epidemics.IMPORTANCEVaccines that can slow respiratory virus transmission in the population are urgently needed for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza virus. Here, we describe how a recombinant neuraminidase-based influenza virus vaccine reduces transmission in vaccinated guinea pigs in an exposure intensity-based manner.