Developmental differences in place-learning performance between C57BL/6 and DBA/2 mice parallel the ontogeny of hippocampal protein kinase C.
Developmental differences in place-learning performance between C57BL/6 and DBA/2 mice parallel the ontogeny of hippocampal protein kinase C.
复制标题
DOI：
10.1037//0735-7044.110.6.1415
复制
发表时间：
1996-12
影响因子：
1.9
通讯作者：
R. Paylor;L. Baskall-Baldini;L. Yuva;J. Wehner
中科院分区：
文献类型：
--
作者：
R. Paylor;L. Baskall-Baldini;L. Yuva;J. Wehner
本研究测定了C57和DBA小鼠学习记忆和海马蛋白激酶C（PKC）的个体发生性变化。在17、24、31或60日龄开始，在可见或隐藏平台版本的Morris水任务上测试小鼠。这两种菌株在所有年龄段都学会了定位可见的平台。C57小鼠在24天大的时候就学会了解决隐藏平台任务，而DBA小鼠从未学会解决这个任务。使用[3 H]-佛波醇酯结合试验，作者发现两种品系在10和17日龄时具有相似数量的海马PKC，但C57小鼠在24，31和60日龄时具有显著更多的PKC。免疫印迹结果显示，C57小鼠比DBA小鼠具有更多的γ-PKC，而不是α-PKC。因此，在C57和DBA小鼠之间的空间学习的性能差异的发展平行海马PKC的个体发育。
This study determined the ontogenic changes in learning and hippocampal protein kinase C (PKC) in C57 and DBA mice. Mice were tested on the visible- or hidden-platform versions of the Morris water task starting at 17, 24, 31, or 60 days of age. Both strains learned to locate the visible platform at all ages. C57 mice learned to solve the hidden-platform task when they were 24 days old, whereas DBA mice never learned to solve this task. Using a [3H]-phorbol ester binding assay, the authors found that both strains had similar amounts of hippocampal PKC at 10 and 17 days of age but that C57 mice had significantly more PKC at 24, 31, and 60 days of age. Immunoblotting results revealed that C57 mice had more gamma-PKC, but not alpha-PKC, than DBA mice. Thus, the development of performance differences in spatial learning between C57 and DBA mice parallels the ontogeny of hippocampal PKC.