Horizontal gene transfers link a human MRSA pathogen to contagious bovine mastitis bacteria.
Horizontal gene transfers link a human MRSA pathogen to contagious bovine mastitis bacteria.
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水平基因将人类MRSA病原体与传染性牛乳腺炎联系起来。
DOI：
10.1371/journal.pone.0003074
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发表时间：
2008-08-27
期刊：
影响因子：
3.7
通讯作者：
Odenwald WF
中科院分区：
文献类型：
--
作者：
Brody T;Yavatkar AS;Lin Y;Ross J;Kuzin A;Kundu M;Fann Y;Odenwald WF
许多临床上重要的细菌获得毒力因子和抗生素耐药性可以追溯到相关或进化上遥远的微生物群之间的水平基因转移（HGT）。比较基因组分析已成为识别新兴病原体中 HGT DNA 的重要工具。我们采用了多基因组比对工具 EvoPrinter，以促进细菌基因组及其移动遗传元件内 HGT DNA 序列的发现。 EvoPrinter 对 13 种不同金黄色葡萄球菌基因组的分析表明，其中一种人类分离株，即医院流行的耐甲氧西林 MRSA252 菌株，与牛乳腺炎的不同病原体独特地共享多个假定的 HGT DNA 序列，而在其他人类金黄色葡萄球菌分离株中未发现这些序列。 MRSA252 与牛乳腺炎 ET3 金黄色葡萄球菌菌株 RF122 共享超过 14 个不同的 DNA 序列块，并且许多 HGT DNA 编码毒力因子。 EvoPrinter 对 MRSA252 染色体的分析还发现，编码 HGT 事件的毒力因子与单核细胞增生李斯特菌的基因组和腐生葡萄球菌相关质粒有关。这两种细菌也是传染性牛乳腺炎的病原体。 EvoPrinter 分析表明，人类 MRSA252 菌株与牛乳腺炎的不同病原体独特地共享多个 DNA 序列块，表明这些病原体之间可能发生 HGT 事件。这些发现对于无意中增强了人类和牲畜细菌病原体接触的畜牧业实践具有重要意义。
Acquisition of virulence factors and antibiotic resistance by many clinically important bacteria can be traced to horizontal gene transfer (HGT) between related or evolutionarily distant microflora. Comparative genomic analysis has become an important tool for identifying HGT DNA in emerging pathogens. We have adapted the multi-genome alignment tool EvoPrinter to facilitate discovery of HGT DNA sequences within bacterial genomes and within their mobile genetic elements. EvoPrinter analysis of 13 different Staphylococcus aureus genomes revealed that one of the human isolates, the hospital epidemic methicillin-resistant MRSA252 strain, uniquely shares multiple putative HGT DNA sequences with different causative agents of bovine mastitis that are not found in the other human S. aureus isolates. MRSA252 shares over 14 different DNA sequence blocks with the bovine mastitis ET3 S. aureus strain RF122, and many of the HGT DNAs encode virulence factors. EvoPrinter analysis of the MRSA252 chromosome also uncovered virulence-factor encoding HGT events with the genome of Listeria monocytogenes and a Staphylococcus saprophyticus associated plasmid. Both bacteria are also causal agents of contagious bovine mastitis. EvoPrinter analysis reveals that the human MRSA252 strain uniquely shares multiple DNA sequence blocks with different causative agents of bovine mastitis, suggesting that HGT events may be occurring between these pathogens. These findings have important implications with regard to animal husbandry practices that inadvertently enhance the contact of human and livestock bacterial pathogens.