Raman spectroscopy for the diagnosis of unlabeled and unstained histopathological tissue specimens.
Raman spectroscopy for the diagnosis of unlabeled and unstained histopathological tissue specimens.
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DOI：
10.4251/wjgo.v10.i11.439
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发表时间：
2018-11-15
影响因子：
3
通讯作者：
Inoue H
中科院分区：
文献类型：
--
作者：
Ikeda H;Ito H;Hikita M;Yamaguchi N;Uragami N;Yokoyama N;Hirota Y;Kushima M;Ajioka Y;Inoue H
探讨利用拉曼光谱技术从未染色的病理组织中诊断胃癌的可能性，并与常规组织病理学检查结果进行比较。我们从一名胃癌患者手术切除的胃中有和没有癌病变的区域连续制备了两份组织标本。两个组织标本之一用苏木精和伊红染色，并用作光谱法激光照射定位的参考。另一个样本保持未染色并用于拉曼光谱分析。在所有的测量点上都可以获得显著的拉曼散射光谱。725 cm-1和782 cm-1的拉曼散射光谱强度分别与核苷酸腺嘌呤和胞嘧啶相关。782 cm-1/620 cm-1、782 cm-1/756 cm-1、782 cm-1/1250 cm-1和782 cm-1的拉曼散射光谱强度比 cm-1/1263 cm-1，胃腺癌组织明显高于正常胃组织。这个初步的实验结果表明，我们的光谱方法作为胃癌的诊断工具，使用未染色的病理标本的可行性。
To investigate the possibility of diagnosing gastric cancer from an unstained pathological tissue using Raman spectroscopy, and to compare the findings to those obtained with conventional histopathology. We produced two consecutive tissue specimens from areas with and without cancer lesions in the surgically resected stomach of a patient with gastric cancer. One of the two tissue specimens was stained with hematoxylin and eosin and used as a reference for laser irradiation positioning by the spectroscopic method. The other specimen was left unstained and used for Raman spectroscopy analysis. A significant Raman scattering spectrum could be obtained at all measurement points. Raman scattering spectrum intensities of 725 cm-1 and 782 cm-1, are associated with the nucleotides adenine and cytosine, respectively. The Raman scattering spectrum intensity ratios of 782 cm-1/620 cm-1, 782 cm-1/756 cm-1, 782 cm-1/1250 cm-1, and 782 cm-1/1263 cm-1 in the gastric adenocarcinoma tissue were significantly higher than those in the normal stomach tissue. The results of this preliminary experiment suggest the feasibility of our spectroscopic method as a diagnostic tool for gastric cancer using unstained pathological specimens.
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影响因子：
3.3
作者：
Winn, Brody;Tavares, Rosemarie;Fanion, Jacqueline;Noble, Lelia;Gao, John;Sabo, Edmond;Resnick, Murray B.
通讯作者：
Resnick, Murray B.
DOI：
10.1016/s1470-2045(17)30438-2
发表时间：
2017-07
期刊：
The Lancet. Oncology
影响因子：
--
作者：
O'Sullivan B;Brierley J;Byrd D;Bosman F;Kehoe S;Kossary C;Piñeros M;Van Eycken E;Weir HK;Gospodarowicz M
通讯作者：
Gospodarowicz M
影响因子：
3.4
作者：
Pence, Isaac J.;Patil, Chetan A.;Mahadevan-Jansen, Anita
通讯作者：
Mahadevan-Jansen, Anita
影响因子：
3.5
作者：
Kong, Kenny;Zaabar, Fazliyana;Notingher, Ioan
通讯作者：
Notingher, Ioan
影响因子：
--
作者：
Liu Y;Du Z;Zhang J;Jiang H
通讯作者：
Jiang H