Dynamics of a Soil Fungal Community in a Three-Year Green Garlic/Cucumber Crop Rotation System in Northwest China
Dynamics of a Soil Fungal Community in a Three-Year Green Garlic/Cucumber Crop Rotation System in Northwest China
复制标题
西北地区大蒜/黄瓜三年青绿轮作系统土壤真菌群落动态
DOI：
10.3390/su10051391
复制
发表时间：
2018-05-01
期刊：
影响因子：
3.9
通讯作者：
Cheng, Zhihui
中科院分区：
文献类型：
--
作者：
Ding, Haiyan;Ali, Ahmad;Cheng, Zhihui
土壤微生物数量和多样性指数的不稳定性是导致土壤生物环境下降的主要原因。在这项研究中，建立了一个作物轮作系统，以评估土壤真菌多样性在连续三个生长季节（2013年，2014年和2015年）在田间盆栽调查使用基于焦磷酸测序的分析。我们发现，整体绿色大蒜/黄瓜轮作效果提高黄瓜生产力下，这些增长的做法。高通量焦磷酸测序对根际土壤真菌群落的分析表明，三年试验期间估计的处理效果更加明显，黄瓜种植土壤中OTU丰富度远高于对照。轮作前后土壤真菌多样性的相对频率表现出不同的响应，主要表现为大蒜种植土壤真菌α多样性增加，黄瓜种植土壤真菌α多样性又下降。系统发育分类表明，真菌群落占主导地位的分类Ascomycota，Basidiomycota，壶菌，球囊菌，接合菌在所有样品。特别是，子囊菌门的相对丰度在很大程度上，主要是丰富的大蒜鳞茎数量的增加，在三年。此外，大多数丰富的类群与有效P和K含量呈正相关，而与土壤pH值，EC和SOC高度负相关。这些结果表明，大蒜黄瓜为基础的作物轮作诱导真菌丰富度和多样性，促进农业生态系统的可持续性，从而提高作物生长和产量。
A decreased soil biological environment in terms of an unstable microbial population and diversity index is primarily caused by intensive continuous cropping systems. In this study, a crop rotation system was established to evaluate the soil fungal diversity using a pyrosequencing-based analysis during three successive growing seasons (2013, 2014, and 2015) under a field pot investigation. We found that overall green garlic/cucumber rotational effects increased cucumber productivity under these growing practices. Analysis of fungal communities in rhizosphere soils by high-throughput pyrosequencing showed that the estimated treatment effects were more obvious during the three-year trials, and OTU richness was much higher than the control in cucumber-planted soils. The relative frequencies of fungal diversity showed variable responses before and after rotation practices, and mainly alpha-diversity of fungi increased in garlic planted soil and again decreased after cucumber planted soil in each year. The phylogenetic classification illustrated that the fungal communities were dominated by the taxa Ascomycota, Basidiomycota, Chytridiomycota, Glomeromycota, and Zygomycota across all samples. Particularly, the relative abundance of the taxon Ascomycota was largely and predominantly enriched with the increasing number of garlic bulbs during the three years. Moreover, the majority of abundant taxa positively correlated with available P and K contents, while being highly negatively correlated with soil pH, EC, and SOC. These results indicate that garlic-cucumber-based crop rotations induce fungal richness and diversity and promote the sustainability of agricultural ecosystems, thus enhancing crop growth and production.