Gestational 1-nitropyrene exposure causes gender-specific impairments on postnatal growth and neurobehavioral development in mice
Gestational 1-nitropyrene exposure causes gender-specific impairments on postnatal growth and neurobehavioral development in mice
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妊娠期 1-硝基芘暴露会对小鼠产后生长和神经行为发育造成性别特异性损害
DOI：
10.1016/j.ecoenv.2019.05.016
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Dexiang Xu
中科院分区：
文献类型：
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作者：
Ran Li;Xilu Wang;Bo Wang;Jian Li;Yaping Song;Biao Luo;Yuanhua Chen;Cheng Zhang;Hua Wang;Dexiang Xu
1-硝基芘（1-NP）是一种典型的硝化多环芳烃，广泛分布于环境中，具有致突变作用。最近，我们发现妊娠期1-NP暴露可导致胎儿生长受限。在这项研究中，我们进一步评估了宫内1-NP暴露对后代出生后生长和神经行为发育的影响。妊娠小鼠在妊娠后期（GD 13-GD 17）每天灌胃给予1-NP（10 μg/kg）。产后1 ~ 12周测量仔鼠体重。产后6周采用旷场实验检测探索和焦虑相关活动。产后7周采用Morris水迷宫评定学习记忆能力。在产后10周时通过蔗糖偏好试验评估抑郁样行为。在PND 1、PND 14和PND 21时，在1-NP暴露的雌性后代中观察到显著的体重降低，而仅在PND 1时观察到1-NP暴露的雄性后代的体重降低。在出生前暴露于1-NP的后代中，青春期的探索和焦虑活动以及成年后的抑郁样行为没有受到干扰。有趣的是，空间学习和记忆能力在青春期受损的女性，但在男性产前暴露于1-NP。这些研究结果表明，妊娠期1-NP暴露延迟出生后的增长和受损的神经行为发育的性别依赖性的方式。
1-Nitropyrene (1-NP), a typical nitrated polycyclic aromatic hydrocarbon, is widely distributed in the environment and is well known for its mutagenic effects. Recently, we found that gestational 1-NP exposure induced fetal growth restriction. In this study, we further evaluated the effect of in utero 1-NP exposure on postnatal growth and neurobehavioral development in the offspring. Pregnant mice were administered with 1-NP (10 μg/kg) by gavage daily in late pregnancy (GD13-GD17). The body weight of each offspring was measured from PND1 to 12 weeks postpartum. Exploration and anxiety related activities were detected by open-field test at 6 weeks postpartum. Learning and memory were assessed by Morris Water Maze at 7 weeks postpartum. And depressive-like behaviors were estimated by sucrose preference test at 10 weeks postpartum. Significant body weight reduction was observed in 1-NP-exposed female offspring at PND1, PND14 and PND21 while the lower body weight was only found at PND1 for 1-NP-exposed male offspring. Exploration and anxiety activities at puberty, and depressive-like behavior in adulthood were not disturbed in offspring prenatally exposed to 1-NP. Interestingly, spatial learning and memory ability at puberty was impaired in females but not in males prenatally exposed to 1-NP. These findings suggest that gestational 1-NP exposure delays postnatal growth and impaired neurobehavioral development in a gender-dependent manner.