METABOLISM OF HEXADECYLTRIMETHYLAMMONIUM CHLORIDE IN PSEUDOMONAS STRAIN-B1
METABOLISM OF HEXADECYLTRIMETHYLAMMONIUM CHLORIDE IN PSEUDOMONAS STRAIN-B1
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DOI：
10.1128/aem.58.9.3083-3087.1992
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发表时间：
1992-09-01
影响因子：
4.4
通讯作者：
KROON, AGM
中科院分区：
文献类型：
--
作者：
VANGINKEL, CG;VANDIJK, JB;KROON, AGM
从活性污泥中分离到一株以十六烷基三甲基氯化铵为碳源和能源的细菌B1，初步鉴定为假单胞菌属。该细菌只生长在烷基三甲基铵盐(C-12至C22)和可能的十六烷基三甲基氯化铵分解的中间体上，如十六酸和醋酸酯。假单胞菌B1菌株不能在胺上生长。同时适应研究表明，细菌只氧化了十六烷基三甲基氯化铵的烷基链。十六烷基三甲基氯化铵形成三甲胺的化学计量比证实了这一点。最初的十六烷基三甲基氯化铵加氧酶活性，通过其形成三甲胺的能力来衡量，依赖于NAD(P)H和O2。细胞提取物中的乙醛脱氢酶、十六酰辅酶A脱氢酶和异柠檬酸裂解酶的测定表明，假单胞菌B1具有通过β-氧化代谢烷基链的能力。
A bacterium (strain B1) utilizing hexadecyltrimethylammonium chloride as a carbon and energy source was isolated from activated sludge and tentatively identified as a Pseudomonas sp. This bacterium only grew on alkyltrimethylammonium salts (C-12 to C22) and possible intermediates of hexadecyltrimethylammonium chloride breakdown such as hexadecanoate and acetate. Pseudomonas strain B1 did not grow on amines. Simultaneous adaptation studies suggested that the bacterium oxidized only the alkyl chain of hexadecyltrimethylammonium chloride. This was confirmed by the stoichiometric formation of trimethylamine from hexadecyltrimethylammonium chloride. The initial hexadecyltrimethylammonium chloride oxygenase activity, measured by its ability to form trimethylamine, was NAD(P)H and O2 dependent. Finally, assays of aldehyde dehydrogenase, hexadecanoyl-coenzyme A dehydrogenase, and isocitrate lyase in cell extracts revealed the potential of Pseudomonas strain B1 to metabolize the alkyl chain via beta-oxidation.