Iron oxide nanoparticles-chitosan composite based glucose biosensor
Iron oxide nanoparticles-chitosan composite based glucose biosensor
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DOI：
10.1016/j.bios.2008.06.032
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发表时间：
2008-12-01
影响因子：
12.6
通讯作者：
Malhotra, B. D.
中科院分区：
文献类型：
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作者：
Kaushika, Ajeet;Khan, Raju;Malhotra, B. D.
采用共沉淀法制备了纳米氧化铁（Fe 3 O 4）分散在壳聚糖（CH）溶液中，并在ITO玻璃上制备了纳米复合膜。利用物理吸附法将葡萄糖氧化酶（GOx）固定在该纳米复合膜上。使用X-射线衍射（XRD）图案和透射电子显微镜（TEM）估计的Fe 3 O 4纳米粒子的大小已被发现是类似于22 nm。采用紫外-可见光谱、傅里叶变换红外光谱和扫描电子显微镜对CH-Fe 3 O 4纳米复合膜和GOx/CH-Fe 3 O 4/ITO生物电极进行了表征。该GOx/CH-Fe 3 O 4/ITO纳米复合生物电极的响应时间为5 s，线性范围为10-400 mg dL（-1）葡萄糖，灵敏度为9.3 μ A/（mg dL cm（2）），在冷藏条件下的保质期约为8周。获得的Michaelis-Menten（Km）常数的值为0.141 mM，表明固定的GOx对底物（葡萄糖）的高亲和力。(C)2008年由Elsevier B. V.出版。
Iron oxide (Fe3O4) nanoparticles prepared using co-precipitation method have been dispersed in chitosan (CH) solution to fabricate nanocomposite film on indium-tin oxide (ITO) glass plate. Glucose oxidase (GOx) has been immobilized onto this CH-Fe3O4 nanocomposite film via physical adsorption. The size of the Fe3O4 nanoparticles estimated using X-ray diffraction (XRD) pattern and transmission electron microscopy (TEM) has been found to be similar to 22 nm. The CH-Fe3O4 nanocomposite film and GOx/CH-Fe3O4/ITO bioelectrode have been characterized using UV-visible and Fourier transform infrared (FTIR) spectroscopic and scanning electron microscopy (SEM) techniques, respectively. This GOx/CH-Fe3O4/ITO nanocomposite bioelectrode has response time of 5 s, linearity as 10-400 mg dL(-1) of glucose, sensitivity as 9.3 mu A/(mg dL cm(2)) and shelf life of about 8 weeks under refrigerated conditions. The value of Michaelis-Menten (Km)constant obtained as 0.141 mM indicates high affinity of immobilized GOx towards the substrate (glucose). (C) 2008 Published by Elsevier B.V.