Soluble Regulatory Proteins for Activation of NOX Family NADPH Oxidases.
Soluble Regulatory Proteins for Activation of NOX Family NADPH Oxidases.
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DOI：
10.1007/978-1-4939-9424-3_8
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发表时间：
2019
影响因子：
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通讯作者：
H. Sumimoto;R. Minakami;K. Miyano
中科院分区：
文献类型：
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作者：
H. Sumimoto;R. Minakami;K. Miyano
NOx家族NADPH氧化酶特意产生活性氧物种，从而发挥多种生物学功能。在人类家族的七个成员中，三个氧化酶NOX2、NOX1和NOX3与p22phoxand形成一个异二聚体，受可溶性调节蛋白的调节：p47Phox，其相关的组织者NOXO1；p67Phox，其相关的激活因子NOXA1；p40Phox；以及小的GTP酶Rac。吞噬细胞氧化酶NOX2的激活需要p47Phox、p67Phox和GTP结合的RAC。除了这些调节因子外，当NOX2在吞噬过程中被激活时，p40phox2也起着至关重要的作用。另一方面，尽管RAC也参与其中，但NOX1的激活更倾向于NOXO1和NOXA1。NOX3结构性地产生超氧化物，这种超氧化物被p47Phox、NOXO1和p67Phox等调节蛋白增强。在这里，我们描述了氮氧化物的激活机制，特别关注了可溶性的调节蛋白。
NOX family NADPH oxidases deliberately produce reactive oxygen species and thus contribute to a variety of biological functions. Of seven members in the human family, the three oxidases NOX2, NOX1, and NOX3 form a heterodimer with p22phoxand are regulated by soluble regulatory proteins: p47phox, its related organizer NOXO1; p67phox, its related activator NOXA1; p40phox; and the small GTPase Rac. Activation of the phagocyte oxidase NOX2 requires p47phox, p67phox, and GTP-bound Rac. In addition to these regulators, p40phoxplays a crucial role when NOX2 is activated during phagocytosis. On the other hand, NOX1 activation prefers NOXO1 and NOXA1, although Rac is also involved. NOX3 constitutively produces superoxide, which is enhanced by regulatory proteins such as p47phox, NOXO1, and p67phox. Here we describe mechanisms for NOX activation with special attention to the soluble regulatory proteins.