Platelet-derived growth factor receptor alpha (pdgfr-α) gene in zebrafish embryonic development
Platelet-derived growth factor receptor alpha (pdgfr-α) gene in zebrafish embryonic development
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DOI：
10.1016/s0925-4773(02)00142-9
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发表时间：
2002-08-01
影响因子：
2.6
通讯作者：
Ge, RW
中科院分区：
文献类型：
--
作者：
Liu, LH;Chong, SW;Ge, RW
本文研究了斑马鱼pdgfr- α全长cDNA的克隆以及该基因在斑马鱼胚胎发生过程中的表达。我们发现斑马鱼的pdgfr-a mRNA在受精卵以及直至原肠胚形成结束的所有胚胎细胞中都存在高水平。该基因的空间限制性表达始于分节开始后，主要定位于前迁移神经嵴细胞、基板细胞、体突前旁轴细胞和尾芽近轴细胞。该基因的瞬时表达也在早期库普弗氏囊泡（一种硬骨鱼特有的结构）中被检测到。与其他脊椎动物相比，斑马鱼pdgfr-a的表达既保守又分化。(C) 2002爱思唯尔科学爱尔兰有限公司版权所有。
Here we present the cloning of a full-length zebrafish pdgfr-alpha cDNA as well as the expression of this gene during zebrafish embryogenesis. We show that zebrafish pdgfr-a mRNA is present at high levels in the fertilized egg as well as in all embryonic cells up to the end of gastrulation. Spatially restricted expression of the gene started after the onset of segmentation and is mainly localized in premigratory neural crest cells, the placodes, the anterior paraxial cells of somites and the adaxial cells of the tailbud. Transient expression of this gene was also detected in the early Kupffer's vesicle, a teleost-specific structure. Expression of the zebrafish pdgfr-a is both conserved as well as diverged comparing to that of other vertebrate species. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.