The leukemic oncogene tal-2 is expressed in the developing mouse brain
The leukemic oncogene tal-2 is expressed in the developing mouse brain
复制标题
DOI：
10.1016/s0169-328x(98)00323-4
复制
发表时间：
1999-02-05
期刊：
影响因子：
--
通讯作者：
Yokota, Y
中科院分区：
文献类型：
--
作者：
Mori, S;Sugawara, S;Yokota, Y
tal-1（T细胞急性白血病-1；也称为SCL）和tal-2基因属于碱性螺旋-环-螺旋转录因子家族，最初是从人类T细胞白血病细胞系的染色体易位断点中分离出来的，tal-1不仅在造血细胞中表达，而且在发育过程中在多种内皮结构和中枢神经系统中表达。另一方面，tal-2的详细功能和表达位点仍然不清楚。我们从小鼠胚胎 cDNA 文库中克隆了 tal-2 cDNA，并检查了其在小鼠中的表达模式，并与 tal-1 进行了比较。原位分析显示，在胚胎第 12.5 天，在以下区域检测到了 tal-2 转录物： 1) 间脑 - 丘脑内限制带和前顶盖，2) 中脑 - 顶盖和前被盖和后被盖，3) 后脑 - 峡部和前笔。在间脑和中脑中，tal-2基因的表达位点与tal-1相似，且表达强于tal-1。在中脑中，在前笔中观察到tal-2表达，而在整个笔中检测到tal-1转录本，并且表现出比tal-2更强的表达。出生时大脑中几乎检测不到 tal-2 信息。这些结果表明tal-1和tal-2参与中枢神经系统特定区域的发育。 (C) 1999 Elsevier Science B.V. 保留所有权利。
tal-1 (T-cell acute leukemia-1; also known as SCL) and tal-2 genes belong to a family of basic helix-loop-helix transcription factors and were originally isolated from the breakpoints of chromosomal translocations in human T-cell leukemia cell lines, tal-1 is expressed not only in hematopoietic cells but also in several endothelial structures and the central nervous system during development. On the other hand, the detailed function and the sites of expression of tal-2 have remained obscure. We cloned the tal-2 cDNA from a mouse embryonic cDNA library and examined its expression pattern in the mouse, comparing with that of tal-1. In situ analyses revealed that tal-2 transcripts are detected at embryonic day 12.5 in the following regions; 1) the diencephalon-the zona limitans intrathalamica and the pretectum, 2) the mesencephalon-the tectum, and the anterior and posterior tegmentum, 3) the metencephalon-the isthmus and the anterior pens. In the diencephalon and the mesencephalon, the expression sites of tal-2 gene were similar to those of tal-1, and its expression was stronger than that of tal-1. In the metencephalon, tal-2 expression was observed in the anterior pens, whereas tal-1 transcripts were detected in the entire pens, and showed stronger expression than tal-2. The tal-2 messages were barely detectable in the brain at birth. These results suggest that tal-1 and tal-2 are involved in the development of specific areas of the central nervous system. (C) 1999 Elsevier Science B.V. All rights reserved.