Single-walled carbon nanotubes based quenching of free FAM-aptamer for selective determination of ochratoxin A
Single-walled carbon nanotubes based quenching of free FAM-aptamer for selective determination of ochratoxin A
复制标题
基于单壁碳纳米管的游离 FAM 适体淬灭用于选择性测定赭曲霉毒素 A
DOI：
10.1016/j.talanta.2011.08.015
复制
发表时间：
2011-10-15
期刊：
影响因子：
6.1
通讯作者：
Wang, Erkang
中科院分区：
文献类型：
--
作者：
Guo, Zhijun;Ren, Jiangtao;Wang, Erkang
赭曲霉毒素 A 是由赭曲霉和疣状青霉产生的毒素，是世界上最丰富的食品污染霉菌毒素之一。它已被国际癌症研究机构（IARC）列为可能的人类致癌物。本文构建了一种用于赭曲霉毒素A（OTA）检测的灵敏、选择性荧光适体传感器，利用单壁碳纳米管（SWNT）作为猝灭剂，可以猝灭附着有FAM（羧基荧光素）的游离未折叠毒素特异性适体的荧光。与基于氧化石墨烯的传感器相比，在没有任何涂层材料的情况下，我们基于 SWNT 的传感平台的检测限为 24.1 nM，线性检测范围为 25 nM 至 200 nM。该技术专门针对 OTA，不受其他类似物（N-乙酰基-L-苯丙氨酸、华法林和 OTB）的干扰。通过测试含有一系列浓度 OTA 的缓冲溶液的 1% 啤酒，也验证了其实际样品应用。 (C) 2011 Elsevier B.V. 保留所有权利。
Ochratoxin A, a toxin produced by Aspergillus ochraceus and Penicillium verrucosum, is one of the most abundant food-contaminating mycotoxins in the world. It has been classified by the International Agency for Research on Cancer (IARC) as a possible human carcinogen. In this paper, a sensitive and selective fluorescent aptasensor for ochratoxin A (OTA) detection was constructed, utilizing single-walled carbon nanotubes (SWNTs) as quencher which can quench the fluorescence of free unfolded toxin-specific aptamer attached with FAM (carboxyfluorescein). Without any coating materials as compared to graphene-oxide based sensor, we obtained the detection limit of our sensing platform based on SWNTs to be 24.1 nM with a linear detection range from 25 nM to 200 nM. This technique responded specifically to OTA without interference from other analogues (N-acetyl-L-phenylalanine, warfarin and OTB). It has also been verified for real sample application by testing 1% beer containing buffer solution spiked with a series of concentration of OTA. (C) 2011 Elsevier B.V. All rights reserved.