PULSATILE RELEASE OF HISTAMINE IN THE HYPOTHALAMUS OF CONSCIOUS RATS
PULSATILE RELEASE OF HISTAMINE IN THE HYPOTHALAMUS OF CONSCIOUS RATS
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DOI：
10.1016/0165-1838(92)90050-q
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发表时间：
1992-06-15
期刊：
影响因子：
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通讯作者：
PHILIPPU, A
中科院分区：
文献类型：
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作者：
PRAST, H;DIETL, H;PHILIPPU, A
在清醒、自由活动的大鼠下丘脑中研究组胺释放模式超过20小时。在麻醉状态下，在下丘脑后部立体定向插入引导套管。在有意识的动物中，用推拉式插管代替导管的管突，下丘脑灌注人工脑脊液。用放射酶法测定过浓液中的组胺含量，每隔20分钟收集一次。组胺的释放率根据超昼夜节律（频率：每83分钟1个周期）和昼夜节律波动，组胺的最高释放率在晚上11点至凌晨1点之间。组胺在黑暗期的释放率高于光照期。结果表明，在大脑中，神经元组胺是根据不同频率的节奏释放的。
The pattern of histamine release was investigated in the hypothalamus of the conscious, freely moving rat over 20 h. Under anaesthesia, a guide cannula was stereotaxically inserted into the posterior hypothalamus. In the conscious animal, the stylet of the guide cannula was replaced by a push-pull cannula, and the hypothalamus was superfused with artificial cerebrospinal fluid. Histamine was determined radioenzymatically in the superfusate which was continuously collected in time periods of 20 min. The release rate of histamine fluctuated according to an ultradian rhythm (frequency: 1 cycle per 83 min) and a circadian rhythm with the highest release rate of histamine between 11.00 p.m. and 1.00 a.m. The release rate of histamine during darkness was higher than that during the light period. The results demonstrate that, in the brain, neuronal histamine is released according to rhythms with various frequencies.