Active site opening and closure control translocation of multisubunit RNA polymerase.
Active site opening and closure control translocation of multisubunit RNA polymerase.
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DOI：
10.1093/nar/gks383
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发表时间：
2012-08
影响因子：
14.9
通讯作者：
Belogurov GA
中科院分区：
文献类型：
--
作者：
Malinen AM;Turtola M;Parthiban M;Vainonen L;Johnson MS;Belogurov GA
多亚基RNA聚合酶（RNAP）是所有细胞生命形式中的中心信息处理酶，但其沿着DNA分子移位的机制尚不清楚。在这里，我们报告直接监测细菌RNAP易位后，添加一个核苷酸。时间分辨的测量表明，易位延迟相对于核苷酸掺入和发生后不久或同时焦磷酸释放。易位平衡的研究表明，RNA 3′核苷酸与亲核位点和底物位点的相互作用强度决定了转录延伸复合物的易位状态，而活性位点的开放和关闭调节底物位点的亲和力，从而分别有利于易位后和易位前的状态。RNAP易位机制被抗生素Tagetitoxin利用，其模拟焦磷酸并通过关闭活性位点诱导反向易位。
Multisubunit RNA polymerase (RNAP) is the central information-processing enzyme in all cellular life forms, yet its mechanism of translocation along the DNA molecule remains conjectural. Here, we report direct monitoring of bacterial RNAP translocation following the addition of a single nucleotide. Time-resolved measurements demonstrated that translocation is delayed relative to nucleotide incorporation and occurs shortly after or concurrently with pyrophosphate release. An investigation of translocation equilibrium suggested that the strength of interactions between RNA 3′ nucleotide and nucleophilic and substrate sites determines the translocation state of transcription elongation complexes, whereas active site opening and closure modulate the affinity of the substrate site, thereby favoring the post- and pre-translocated states, respectively. The RNAP translocation mechanism is exploited by the antibiotic tagetitoxin, which mimics pyrophosphate and induces backward translocation by closing the active site.
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影响因子：
16.6
作者：
Nudler E
通讯作者：
Nudler E
影响因子：
11.4
作者：
Berman, Andrea J.;Kamtekar, Satwik;Steitz, Thomas A.
通讯作者：
Steitz, Thomas A.
影响因子：
4.8
作者：
Marchand, Bruno;Tchesnokov, Egor P.;Gotte, Matthias
通讯作者：
Gotte, Matthias
DOI：
10.1073/pnas.1012277107
发表时间：
2010-10-19
影响因子：
11.1
作者：
Datta, Kausiki;Johnson, Neil P.;von Hippel, Peter H.
通讯作者：
von Hippel, Peter H.
影响因子：
16.8
作者：
Brueckner, Florian;Cramer, Patrick
通讯作者：
Cramer, Patrick