Regulation of factors controlling angiogenesis in liver development: a role for PEDF in the formation and maintenance of normal vasculature
Regulation of factors controlling angiogenesis in liver development: a role for PEDF in the formation and maintenance of normal vasculature
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DOI：
10.1016/j.bbrc.2004.10.041
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发表时间：
2004-12-10
影响因子：
3.1
通讯作者：
Tombran-Tink, J
中科院分区：
文献类型：
--
作者：
Sawant, S;Aparicio, S;Tombran-Tink, J
PEDF和VEGF是血管生成的重要抑制剂和促进剂，两者的比值是许多新生血管疾病的重要指标。在小鼠肝脏中，PEDF 和 VEGF(165) 在肝脏发育的早期阶段共表达，并且随着肝脏胚胎发生的进展，它们的表达增加，表明 PEDF 和 VEGF 对血管发生也至关重要。 VEGF 189 仅出现在肝器官发生的 PO 阶段，此后维持在高水平。 PEDF 和两种 VEGF 同工型由新鲜和培养的肝细胞合成。 VEGF(121) 表达和 VEGF165 过表达仅在 HepG2（一种充分表征的肝细胞癌细胞系）中观察到。结果表明，肝血管结构受到PEDF和VEGF的控制，并且VEGF165和VEGF(189)在肝脏正常血管发育中具有不同的功能。 VEGF 亚型 121 和 189 可能是肝细胞癌（最常见的恶性肿瘤之一）血管分布增加和进展的关键调节因子，并且可能对该肿瘤具有预后意义。 (C) 2004 Elsevier Inc. 保留所有权利。
PEDF and VEGF are important inhibitors and promoters of angiogenesis, and the ratio between the two is an important indicator in many neovascular diseases. In mouse liver PEDF and VEGF(165) were co-expressed at very early stages of liver development and their expression increased as liver embryogenesis progressed, suggesting that PEDF and VEGF are both crucial to vasculogenesis as well. VEGF 189 only appears at the PO stage in liver organogenesis and is maintained at high levels thereafter. PEDF and the two VEGF isoforms are synthesized by fresh and cultured hepatocytes. Expression of VEGF(121) and overexpression of VEGF165 were only seen in HepG2, a well-characterized hepatocellular carcinoma line. The results suggest that hepatic vascular architecture is under the control of both PEDF and VEGF, and that VEGF165 and VEGF(189) have distinct functions in normal vascular development of the liver. The VEGF isoforms 121 and 189 may be key regulators of increased vascularity and progression of hepatocellular carcinoma, one of the most common malignant tumors, and may be of prognostic significance for this tumor. (C) 2004 Elsevier Inc. All rights reserved.