Purification of cytoplasmic tubulin and microtubule organizing center proteins functioning in microtubule initiation from the alga Polytomella.
Purification of cytoplasmic tubulin and microtubule organizing center proteins functioning in microtubule initiation from the alga Polytomella.
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纯化在藻类 Polytomella 的微管起始中起作用的细胞质微管蛋白和微管组织中心蛋白。
DOI：
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发表时间：
1979
影响因子：
11.1
通讯作者：
D. Brown
中科院分区：
文献类型：
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作者：
M. Stearns;D. Brown
本文报道了鞭毛虫Polytomella的细胞质微管蛋白和细胞骨架微管系统的微管组织中心（MTOC）的分离。分离的MTOC作为纯化的微管蛋白体外组装的位点。在该组装中起作用的主要蛋白质（分子量为190，000 - 210，000的四种多肽）已从MTOC中提取并纯化。用体内35 S标记的MTOC蛋白（或35 S标记的微管蛋白）进行的动力学研究和实验表明，这些蛋白质在微管起始中特异性地起作用，并且不有助于微管伸长。结果表明，微管组装在体内是由微管启动蛋白与细胞器称为MTOC控制。
Cytoplasmic tubulin and the microtubule organizing centers (MTOCs) for the cytoskeletal microtubule system of the flagellate Polytomella have been isolated. The isolated MTOCs serve as sites for the in vitro assembly of the purified tubulin protein. The major proteins (four polypeptides of molecular weights 190,000-210,000) functioning in this assembly have been extracted from the MTOCs and purified. Kinetic studies and experiments with in vivo 35S-labeled MTOC proteins (or 35S-labeled tubulin) demonstrate that these proteins function specifically in microtubule initiation and do not contribute to microtubule elongation. The results indicate that microtubule assembly in vivo is controlled by microtubule initiating proteins associated with the organelles termed MTOCs.