Spatial organization of EGF receptor transmodulation by PDGF
Spatial organization of EGF receptor transmodulation by PDGF
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DOI：
10.1006/bbrc.1999.1082
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发表时间：
1999-08-11
影响因子：
3.1
通讯作者：
Anderson, RGW
中科院分区：
文献类型：
--
作者：
Liu, PS;Anderson, RGW
尽管PDGF对EGF受体的调节已被充分记录，但尚不清楚这两种受体系统之间的串扰发生在细胞表面的哪个位置。最近的研究发现，这两种受体都集中在细胞表面的小泡中，这表明将这两种受体限制在这个空间可能有助于它们的相互作用。在这里，我们发现用PDGF刺激小泡中的PDGF受体会导致相同膜部分的EGF受体亚群在酪氨酸上磷酸化，与酪氨酸磷酸化一致，EGF与其受体的结合明显下降。酪氨酸激酶抑制剂可部分阻断EGF结合的丧失。尽管这两种受体在小囊中非常接近，但我们没有看到EGF可以刺激PDGFR酪氨酸磷酸化的证据。这些结果表明，这两种受体系统在小泡中高度组织。(C) 1999学术出版社。
Even though the modulation of EGF receptors by PDGF is well documented, it is not known where on the cell surface cross-talk between the two receptor systems takes place. The recent finding that both populations of receptors are concentrated in cell surface caveolae suggests that the confinement of the two receptors to this space might facilitate their interaction. Here we show that stimulation of PDGF receptors in caveolae with PDGF causes a subpopulation of EGF receptors in the same membrane fraction to become phosphorylated on tyrosine, Coincident with tyrosine phosphorylation, the binding of EGF to its receptor markedly declines. Loss of EGF binding is partially blocked by tyrosine kinase inhibitors. Despite the close proximity of the two receptors in caveolae, we saw no evidence that EGF could stimulated PDGFR tyrosine phosphorylation. These results suggest that these two receptor systems are highly organized in caveolae. (C) 1999 Academic Press.