Markedly elevated serum MMP-9 (gelatinase B) levels in rheumatoid arthritis: A potentially useful laboratory marker
Markedly elevated serum MMP-9 (gelatinase B) levels in rheumatoid arthritis: A potentially useful laboratory marker
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DOI：
10.1006/clin.1996.0025
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发表时间：
1996-02-01
期刊：
影响因子：
--
通讯作者：
Arbeit, LA
中科院分区：
文献类型：
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作者：
Gruber, BL;Sorbi, D;Arbeit, LA
在试图找到一个潜在的有用的血清标志物在类风湿关节炎（RA），反映潜在的致病机制，我们测量了循环水平的基质降解金属蛋白酶-9（MMP-9），也称为明胶酶B，在血清和滑液（SF）从RA患者，也定量的沉积和局部合成的MMP-9在RA滑膜。临床样本，明胶底物酶谱，抗原免疫测定，和定量底物降解试验，发现升高92-和72-kDa的酶原形式的MMP-9和MMP-2在RA血清和SF与健康对照组相比。新鲜RA滑膜标本的免疫组化染色显示MMP-9在血管壁成纤维细胞样细胞和巨噬细胞; mRNA合成采用逆转录酶原位PCR检测。总之，MMP-9水平在RA患者的血清和SF中显著升高。RA滑膜是这种MMP-9产生的来源，在几种不同类型的类风湿性滑膜细胞中观察到丰富的mRNA和蛋白质。(C)出版社：Academic Press，Inc.
In an attempt to find a potentially useful serum marker in rheumatoid arthritis (RA) which reflects underlying pathogenic mechanisms, we measured the circulating levels of matrix-degrading metalloproteinase-9 (MMP-9), also termed gelatinase B, in sera and synovial fluid (SF) from patients with RA and also quantitated the deposition and local synthesis of MMP-9 in RA synovium. Clinical samples, subjected to gelatin substrate zymography, antigenic immunoassay, and a quantitative substrate degradation assay, revealed elevated 92- and 72-kDa proenzyme forms of MMP-9 and MMP-2 in RA sera and SF compared with healthy controls. Immunostaining on fresh RA synovial specimens revealed MMP-9 within vascular walls in fibroblast-like cells and macrophages; mRNA synthesis was detected using reverse transcriptase in situ PCR. In summary, MMP-9 levels are substantially elevated in the sera and SF from patients with RA. The RA synovium is a source of this MMP-9 production, with abundant mRNA and protein observed within several different type of rheumatoid synovial cells. (C) 1996 Academic Press, Inc.