Comparison of the biodistribution of two hypoxia markers [18F]FETNIM and [18F]FMISO in an experimental mammary carcinoma
Comparison of the biodistribution of two hypoxia markers [18F]FETNIM and [18F]FMISO in an experimental mammary carcinoma
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DOI：
10.1007/s00259-003-1404-x
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发表时间：
2004-04-01
影响因子：
9.1
通讯作者：
Solin, O
中科院分区：
文献类型：
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作者：
Grönroos, T;Bentzen, L;Solin, O
本研究的首要目的是比较氟 18 氟赤硝基咪唑 ([F-18]FETNIM) 与氟 18 氟异咪唑 ([F-18]FMISO) 在两种不同氧合条件下对不同大小的小鼠肿瘤的缺氧成像能力。其次，我们想要评估相似条件下正常组织中标记物的生物分布。使用背部长有 C3H 乳腺癌的雌性 CDF1 小鼠。肿瘤大小匹配，动物呼吸正常空气（21% O-2）或卡波金气体（95% O-2 + 5% CO2）。在静脉注射[F-18]FETNIM或[F-18]FMISO之前5分钟开始放气程序，并持续到120分钟时处死小鼠。取出血液、肿瘤、肌肉、心脏、肺、肝脏、肾脏和脂肪，计算放射性并称重。将肿瘤和肌肉冷冻并用冷冻切片机切成切片。通过数字放射自显影确定组织切片放射性的空间分布。对福尔马林固定肿瘤的切片进行坏死分数的估计。数字放射自显影显示，对于 [F-18]FETNIM，正常呼吸空气的小鼠的整个肿瘤与肌肉的放射性摄取比显着高于卡波金治疗的小鼠（4.9+/-2.6 vs 1.8+/-0.5；P
The first aim of this study was to compare the hypoxia imaging ability of fluorine-18 fluoroerythronitroimidazole ([F-18]FETNIM) with that of fluorine-18 fluoromisonimidazole ([F-18]FMISO) in murine tumours of different sizes under two different oxygenation conditions. Secondly, we wanted to assess the biodistribution of the markers in normal tissues under similar conditions. Female CDF1 mice with a C3H mammary carcinoma grown on their backs were used. Tumours were size matched and animals breathed either normal air (21% O-2) or carbogen gas (95% O-2 + 5% CO2). The gassing procedure was begun 5 min before the intravenous injection of either [F-18]FETNIM or [F-18]FMISO and continued until the mice were sacrificed at 120 min. Blood, tumour, muscle, heart, lung, liver, kidney and fat were removed, counted for radioactivity and weighed. The tumour and muscle were frozen and cut with a cryomicrotome into sections. The spatial distribution of radioactivity from the tissue sections was determined with digital autoradiography. Estimation of the necrotic fraction was made on sections from formalin-fixed tumours. Digital autoradiography showed that the whole tumour-to-muscle radioactivity uptake ratios were significantly higher in normal air-breathing mice than in carbogen-treated mice for both [F-18]FETNIM (4.9+/-2.6 vs 1.8+/-0.5; P