Revised Mechanism and Improved Efficiency of the QuikChange Site-Directed Mutagenesis Method.
Revised Mechanism and Improved Efficiency of the QuikChange Site-Directed Mutagenesis Method.
复制标题
QuikChange 定点诱变方法的改进机制和效率提高。
作者：
Yongzhen Xia;L. Xun
定点诱变已广泛用于特定 DNA 序列中核苷酸残基的取代、添加或删除。 QuikChange™ 定点诱变及其相关方案由于方便和高效而已广泛用于此目的。我们最近证明 QuikChange™ 定点诱变过程的机制与所提议的不同。新机制促进使用部分重叠的引物和商业PCR酶来实现高效的PCR和诱变。
Site-directed mutagenesis has been widely used for the substitution, addition or deletion of nucleotide residues in a defined DNA sequence. QuikChange™ site-directed mutagenesis and its related protocols have been widely used for this purpose because of convenience and efficiency. We have recently demonstrated that the mechanism of the QuikChange™ site-directed mutagenesis process is different from that being proposed. The new mechanism promotes the use of partially overlapping primers and commercial PCR enzymes for efficient PCR and mutagenesis.