Facile synthesis of a boronate affinity sorbent from mesoporous nanomagnetic polyhedral oligomeric silsesquioxanes composite and its application for enrichment of catecholamines in human urine
Facile synthesis of a boronate affinity sorbent from mesoporous nanomagnetic polyhedral oligomeric silsesquioxanes composite and its application for enrichment of catecholamines in human urine
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由介孔纳米磁性多面体低聚倍半硅氧烷复合材料轻松合成硼酸亲和吸附剂及其在人尿中儿茶酚胺富集中的应用
DOI：
10.1016/j.aca.2016.09.012
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发表时间：
2016-11-09
影响因子：
6.2
通讯作者：
Feng, Yuqi
中科院分区：
文献类型：
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作者：
He, Haibo;Zhou, Ziqing;Feng, Yuqi
以纳米磁性多面体低聚倍半硅氧烷（POSS）复合材料（Fe3O4@POSS）为基础平台，轻松合成了硼酸盐修饰的纳米磁性有机-无机杂化材料。 3-丙烯酰氨基苯基硼酸（AAPBA）和底物提供的残留末端乙烯基之间发生简单的共聚。这里特别强调的是八乙烯基 POSS，它不仅充当混合结构的构建块，而且还促进了将硼酸酯基团接枝到 POSS 基纳米磁性复合材料 (Fe3O4@POSS) 表面的过程。通过傅里叶变换红外(FT-IR)、元素分析、电感耦合等离子体原子发射光谱仪(ICP-AES)、场发射扫描电子显微镜证实了亲和配体-AAPBA在Fe3O4@POSS上的成功固定。使用所制备的 Fe3O4@POSS-AAPBA 材料作为亲和吸附剂和三种儿茶酚胺 (CA)，即去甲肾上腺素、肾上腺素和异丙肾上腺素作为模型分析物，开发了用于顺式二醇富集的磁固相萃取 (MSPE)。在最佳提取条件下，通过高效液相色谱紫外检测器（HPLC-UV）实现了对尿液样品中三种CA的灵敏同步分析。目标分析物的检测限（LOD，S/N = 3）和定量限（LOQ，S/N = 10）分别为0.81-1.32 ng mL(-1)和2.70-4.40 ng mL(-1)。还具有良好的回收率 (85.5-101.7%) 和重复性 (RSD
A boronate-decorated nanomagnetic organic-inorganic hybrid material was facilely synthesized by utilizing the nanomagnetic polyhedral oligomeric silsesquioxanes (POSS) composite (Fe3O4@POSS) as the base platform. A simple copolymerization occurred between 3-acrylamidophenylboronic acid (AAPBA) and the residual end vinyl groups supplied by the substrate. Here the special emphasis was placed on the octavinyl POSS, which not only acted as the building blocks for a hybrid architecture but also facilitated the process of grafting boronate groups onto the surface of POSS based nanomagnetic composite (Fe3O4@POSS). The successful immobilization of affinity ligand-AAPBA on the Fe3O4@POSS was confirmed by Fourier transform infrared (FT-IR), elemental analysis, inductively coupled plasma atomic emission spectrometer (ICP-AES), field emission scanning electron microscope. A magnetic solid-phase extraction (MSPE) for cis-diols enrichment was developed using the as-prepared Fe3O4@POSS-AAPBA material as an affinity sorbent and three catecholamines (CAs), namely noradrenaline, epinephrine and isoprenaline, as model analytes. Under the optimal extraction conditions, sensitive and simultaneous analysis of three CAs from the urine sample was achieved by high-performance liquid chromatography with UV detection (HPLC-UV). The limits of detection (LOD, S/N = 3) and the limits of quantitation (LOQ, S/N = 10) for the target analytes were 0.81-1.32 ng mL(-1) and 2.70-4.40 ng mL(-1), respectively. Also good recoveries (85.5-101.7%) and repeatability (RSD