RANKL-induced M1 macrophages are involved in bone formation.
RANKL-induced M1 macrophages are involved in bone formation.
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DOI：
10.1038/boneres.2017.19
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发表时间：
2017
期刊：
影响因子：
12.7
通讯作者：
Xiao Y
中科院分区：
文献类型：
--
作者：
Huang R;Wang X;Zhou Y;Xiao Y
M1巨噬细胞的活化可以通过用脂多糖（LPS）和干扰素-γ（IFN-γ）刺激它们来实现。然而，M1可以在没有任何病理刺激的生理条件下被发现。本研究旨在了解RANKL诱导的M1巨噬细胞与病理诱导的巨噬细胞相比参与骨形成。采用Fischer大鼠，观察正常和损伤股骨髁内巨噬细胞的分布。用LPS+IFN-γ和RANKL激活骨髓源性巨噬细胞（BMDM），以实现体外M1激活。通过逆转录-定量聚合酶链反应（RT-qPCR）和荧光激活细胞分选（FACS）测定与炎症、破骨细胞生成、血管生成和迁移相关的基因表达。组织巨噬细胞在不同的骨区域表现出不同的表达模式。在体内发现RANKL与诱导型一氧化氮合酶阳性（iNOS+）细胞非常接近，表明RANKL表达与iNOS+细胞之间存在相关性，尤其是在骨小梁中。与病理诱导的巨噬细胞相比，RANKL诱导的巨噬细胞显示出不同的细胞因子分泌特征。破骨细胞和M1巨噬细胞在骨愈合过程中均在第7天达到峰值。RANKL可激活M1样巨噬细胞，其性质与LPS+IFN-γ诱导的巨噬细胞不同。这些RANKL激活的M1巨噬细胞积极参与骨形成。
The activation of M1 macrophages can be achieved by stimulating them with lipopolysaccharide (LPS) and interferon-γ (IFN-γ). However, M1 can be found under physiological conditions without any pathological stimuli. This study aimed to understand the involvement of RANKL-induced M1 macrophages in bone formation compared with pathologically induced macrophages. Fischer rats were used to investigate macrophage distribution in normal and injured femoral condyles in vivo. Bone marrow-derived macrophages (BMDMs) were activated with LPS+IFN-γ and RANKL to achieve M1 activation in vitro. Gene expression related to inflammation, osteoclastogenesis, angiogenesis, and migration was determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and fluorescence-activated cell sorting (FACS). Tissue macrophages showed distinct expression patterns at different bone regions. RANKL was found in close proximity to inducible nitric oxide synthase-positive (iNOS+) cells in vivo, suggesting an association between RANKL expression and iNOS+ cells, especially in trabecular bone. RANKL-induced macrophages showed a different cytokine secretion profile compared with pathologically induced macrophages. Both osteoclasts and M1 macrophages peaked on day 7 during bone healing. RANKL could trigger M1-like macrophages with properties that were different from those of LPS+IFN-γ-induced macrophages. These RANKL-activated M1 macrophages were actively involved in bone formation.
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影响因子：
3.7
作者：
Jablonski KA;Amici SA;Webb LM;Ruiz-Rosado Jde D;Popovich PG;Partida-Sanchez S;Guerau-de-Arellano M
通讯作者：
Guerau-de-Arellano M
影响因子：
9.3
作者：
Cherry JD;Olschowka JA;O'Banion MK
通讯作者：
O'Banion MK
DOI：
10.1084/jem.176.1.287
发表时间：
1992-07-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Stein M;Keshav S;Harris N;Gordon S
通讯作者：
Gordon S
影响因子：
6.2
作者：
Alexander, Kylie A.;Chang, Ming K.;Pettit, Allison R.
通讯作者：
Pettit, Allison R.
影响因子：
7.5
作者：
Loi F;Córdova LA;Zhang R;Pajarinen J;Lin TH;Goodman SB;Yao Z
通讯作者：
Yao Z